Difference between revisions of "Rny"
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=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' | + | * '''Mutant:''' essential!!!!, 4043 (''rny'' under p-spac control), GP193 (''rny'' under p-xyl control), available in [[Stülke]] lab |
| − | * '''Expression vector:''' | + | * '''Expression vector:''' pGP441(N-terminal Strep-tag, in [[pGP172]]), pGP755 (N-terminal Strep-tag, for SPINE, expression in ''B. subtilis'', in [[pGP380]]), available in [[Stülke]] lab |
| − | * '''lacZ fusion:''' | + | * '''lacZ fusion:''' pGP459 (in [[pAC7]]) |
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
| − | * '''Antibody:''' | + | * '''Antibody:''' available in [[van Dijl]] and [[Stülke]] labs |
=Labs working on this gene/protein= | =Labs working on this gene/protein= | ||
Revision as of 20:16, 14 January 2009
- Description: required for the processing of the gapA operon mRNA
| Gene name | rny |
| Synonyms | ymdA |
| Essential | yes |
| Product | 2',3'-cyclic-nucleotide 2'-phosphodiesterase |
| Function | required for the processing of the gapA operon mRNA |
| MW, pI | 58,7 kDa, 5.39 |
| Gene length, protein length | 1560 bp, 520 amino acids |
| Immediate neighbours | pdbX, ymdB |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
| |
Contents
The gene
Basic information
- Coordinates: 1766580 - 1768139
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry:
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: required for the processing of the gapA operon mRNA
- Protein family: 2',3' cyclic nucleotide phosphodiesterase family
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- transmembrane domain (4–24)
- KH domain (210–273)
- HD domain (336–429)
- Modification:
- Cofactor(s): metal-dependent
- Effectors of protein activity:
- Interactions:
- Localization: Single-pass membrane protein
Database entries
- Structure:
- Swiss prot entry: [2]
- KEGG entry: [3]
- E.C. number: [4]
Additional information
required for the processing of the gapA operon mRNA
Expression and regulation
- Sigma factor:
- Regulation: constitutive
- Regulatory mechanism:
- Additional information: there is a terminator between rny and ymdB, most transcripts terminate there
Biological materials
- Mutant: essential!!!!, 4043 (rny under p-spac control), GP193 (rny under p-xyl control), available in Stülke lab
- Expression vector: pGP441(N-terminal Strep-tag, in pGP172), pGP755 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Stülke lab
- lacZ fusion: pGP459 (in pAC7)
- GFP fusion:
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany Homepage
Your additional remarks
References
- Commichau, F. M., Rothe, F. M., Herzberg, C., Wagner, E., Hellwig, D., Lehnik-Habrink, M., Hammer, E., Völker, U. & Stülke, J. Novel activities of glycolytic enzymes in Bacillus subtilis: Interactions with essential proteins involved in mRNA processing. subm.
- Hunt, A., Rawlins, J. P., Thomaides, H. B., and Errington, J. (2006) Functional analysis of 11 putative essential genes in Bacillus subtilis. Microbiology 152, 2895-2907. PubMed
