Difference between revisions of "PdaB"
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|style="background:#ABCDEF;" align="center"|'''Function''' || spore cortex formation | |style="background:#ABCDEF;" align="center"|'''Function''' || spore cortex formation | ||
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| + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU01570 pdaB] | ||
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 9.182 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 9.182 | ||
Revision as of 14:30, 6 August 2012
| Gene name | pdaB |
| Synonyms | ybaN |
| Essential | no |
| Product | polysaccharide deacetylase |
| Function | spore cortex formation |
| Gene expression levels in SubtiExpress: pdaB | |
| MW, pI | 28 kDa, 9.182 |
| Gene length, protein length | 762 bp, 254 aa |
| Immediate neighbours | kbaA, rrnI-16S |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU01570
Phenotypes of a mutant
pdaB ytrI and pdaB ytrH mutants are deficient in sporulation
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: polysaccharide deacetylase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: forespore (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P50865
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: pdaB PubMed
- Regulation: expressed during sporulation in the mother cell PubMed
- Additional information: the mRNA is very stable (half-life > 15 min) PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Tatsuya Fukushima, Taichi Tanabe, Hiroki Yamamoto, Shigeo Hosoya, Tsutomu Sato, Hirofumi Yoshikawa, Junichi Sekiguchi
Characterization of a polysaccharide deacetylase gene homologue (pdaB) on sporulation of Bacillus subtilis.
J Biochem: 2004, 136(3);283-91
[PubMed:15598884]
[WorldCat.org]
[DOI]
(P p)
Jessica M Silvaggi, David L Popham, Adam Driks, Patrick Eichenberger, Richard Losick
Unmasking novel sporulation genes in Bacillus subtilis.
J Bacteriol: 2004, 186(23);8089-95
[PubMed:15547282]
[WorldCat.org]
[DOI]
(P p)
G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008]
[WorldCat.org]
[DOI]
(P p)
Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922]
[WorldCat.org]
[DOI]
(P p)
