ComA

• Description: two-component system response regulator, controls gene expression in response to cell density (concentration of ComX)
  
  

Gene name	comA
Synonyms	srfB, comAA
Essential	no
Product	two-component system response regulator
Function	regulation of genetic competence and quorum sensing
Interactions involving this protein in SubtInteract: ComA
MW, pI	23 kDa, 4.759
Gene length, protein length	642 bp, 214 aa
Immediate neighbours	yuxO, comP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

genetic competence, transcription factors and their control, quorum sensing, phosphoproteins

The gene

Basic information

• Locus tag: BSU31680

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ComA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated by ComP on an Asp residue

• Cofactor(s):

• Effectors of protein activity:
  • phosphorylation activates DNA binding
  • DNA-binding activity of ComA is inhibited by RapC PubMed, RapD PubMed, RapF PubMed, RapH PubMed and RapK PubMed

• Interactions:
  • RapC-ComA PubMed
  • RapF-ComA PubMed
  • RapH-ComA PubMed
  • ComP-ComA

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:
  • 1U83
  • 3ULQ (structure of the complex between the DNA-binding C-terminal domain of ComA with RapF) PubMed

• UniProt: P14204

• KEGG entry: [3]

• E.C. number: 4.4.1.19

Expression and regulation

• Operon: comA-yuxO PubMed

• Expression browser: comA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Daniel López, Roberto Kolter
Extracellular signals that define distinct and coexisting cell fates in Bacillus subtilis.
FEMS Microbiol Rev: 2010, 34(2);134-49
[PubMed:20030732] [WorldCat.org] [DOI] (I p)

Leendert W Hamoen, Gerard Venema, Oscar P Kuipers
Controlling competence in Bacillus subtilis: shared use of regulators.
Microbiology (Reading): 2003, 149(Pt 1);9-17
[PubMed:12576575] [WorldCat.org] [DOI] (P p)