SerA

• Description: phosphoglycerate dehydrogenase
  
  

Gene name	serA
Synonyms
Essential	no
Product	phosphoglycerate dehydrogenase
Function	biosynthesis of serine
Metabolic function and regulation of this protein in SubtiPathways: Ala, Gly, Ser
MW, pI	56 kDa, 5.617
Gene length, protein length	1575 bp, 525 aa
Immediate neighbours	ypzE, aroC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, membrane proteins

The gene

Basic information

• Locus tag: BSU23070

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 3-phospho-D-glycerate + NAD⁺ = 3-phosphonooxypyruvate + NADH (according to Swiss-Prot)

• Protein family: D-isomer specific 2-hydroxyacid dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • S-cysteinylation after diamide stress (C410)PubMed
  • S-bacillithiolation upon hypochlorite stress on Cys-410 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane PubMed

Database entries

• Structure: 1YBA (from E. coli, 30% identity, 52% similarity) PubMed

• UniProt: P35136

• KEGG entry: [3]

• E.C. number: 1.1.1.95

Expression and regulation

• Operon: serA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion: pGP187 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody: