CimH

• Description: transporter for citrate and malate (proton symport)
  
  

Gene name	cimH
Synonyms	yxkJ
Essential	no
Product	transporter for citrate and malate (proton symport)
Function	citrate and malate uptake
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	48 kDa, 6.554
Gene length, protein length	1350 bp, 450 aa
Immediate neighbours	cydA, yxkI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

• Locus tag: BSU38770

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: uptake of citrate and malate (symport with protons) PubMed

• Protein family: sodium:citrate (SCF) symporter family (according to Swiss-Prot) 2-hydroxycarboxylate transporter family

• Paralogous protein(s): MaeN

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P94363

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: cimH PubMed

• Expression browser: cimH PubMed

• Sigma factor:

• Regulation:
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed

• Additional information:
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Iwona Sobczak, Juke S Lolkema
The 2-hydroxycarboxylate transporter family: physiology, structure, and mechanism.
Microbiol Mol Biol Rev: 2005, 69(4);665-95
[PubMed:16339740] [WorldCat.org] [DOI] (P p)

Original publications

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947