Difference between revisions of "Spx"
| Line 36: | Line 36: | ||
=== Basic information === | === Basic information === | ||
| − | * '''Coordinates:''' | + | * '''Coordinates:''' |
| + | From To Direction Type | ||
| + | 1227010 1227402 + CDS | ||
| + | 1227427 1227442 + terminator | ||
| + | |||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | |||
| + | Loss of up-regulation of the methionine sulfoxide reductase (''[[mrsA]]-[[mrsB]]'') operon in response to thiol specific oxidative stress, also loss of ''[[trxA]]'' and ''[[trxB]]'' upregulation in response to thiol specific oxidative stress. | ||
=== Database entries === | === Database entries === | ||
| − | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ | + | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spx.html] |
* '''SubtiList entry:''' | * '''SubtiList entry:''' | ||
| Line 53: | Line 59: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' Transcriptional regulator of many genes in response to thiol specific oxidative stress. In addition, Spx inhibits transcription by binding to the C-terminal domain of the alpha subunit of RNAP ([[RpoA]]), disrupting complex formation between RNAP and certain transcriptional activator proteins like [[ResD]] and [[ComA]]. In response to thiol specific oxidative stress, Spx can also activate transcription, making it a general regulator that exerts both positive and negative control over transcription initiation. |
| − | * '''Protein family:''' | + | * '''Protein family:''' Arsenate Reductase (ArsC) family, Spx subfamily |
| − | * '''Paralogous protein(s):''' | + | * '''Paralogous protein(s):''' [[MgsR]] |
=== Extended information on the protein === | === Extended information on the protein === | ||
| Line 63: | Line 69: | ||
* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''Domains:''' CXXC (10-13): Acts as a disulfide switch for the redox-sensitive transcriptional regulation of genes that function in thiol homeostasis. |
| − | * '''Modification:''' | + | * '''Modification:''' Cysteine oxidation of the CXXC motif |
* '''Cofactor(s):''' | * '''Cofactor(s):''' | ||
| Line 71: | Line 77: | ||
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''Interactions:''' | + | * '''Interactions:''' Spx-[[RpoA]] (C-terminal domain) |
* '''Localization:''' | * '''Localization:''' | ||
| Line 90: | Line 96: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''[[yjbC]]-[[spx]]'', ''[[spx]]'' |
| − | * '''Sigma factor:''' | + | * '''Sigma factor:''' four promoters upstream of ''[[yjbC]]'': [[SigW]], [[SigB]], [[SigX]], and unknown sigma |
| + | two promoters upstream of ''spx'': [[SigA]], [[SigW]] | ||
* '''Regulation:''' | * '''Regulation:''' | ||
Revision as of 17:06, 12 January 2009
- Description: Transcriptional regulator Spx, involved in regulation of many genes.
| Gene name | spx |
| Synonyms | yjbD |
| Essential | no |
| Product | transcriptional regulator Spx |
| Function | negative and positive regulator of many genes |
| MW, pI | 15,5 kDa, 7.80 |
| Gene length, protein length | 393 bp, 131 amino acids |
| Immediate neighbours | yjbC, yjbE |
| Gene sequence (+200bp) | Protein sequence |
| Genetic context File:GenE context.gif | |
Contents
The gene
Basic information
- Coordinates:
From To Direction Type 1227010 1227402 + CDS 1227427 1227442 + terminator
Phenotypes of a mutant
Loss of up-regulation of the methionine sulfoxide reductase (mrsA-mrsB) operon in response to thiol specific oxidative stress, also loss of trxA and trxB upregulation in response to thiol specific oxidative stress.
Database entries
- DBTBS entry: [1]
- SubtiList entry:
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Transcriptional regulator of many genes in response to thiol specific oxidative stress. In addition, Spx inhibits transcription by binding to the C-terminal domain of the alpha subunit of RNAP (RpoA), disrupting complex formation between RNAP and certain transcriptional activator proteins like ResD and ComA. In response to thiol specific oxidative stress, Spx can also activate transcription, making it a general regulator that exerts both positive and negative control over transcription initiation.
- Protein family: Arsenate Reductase (ArsC) family, Spx subfamily
- Paralogous protein(s): MgsR
Extended information on the protein
- Kinetic information:
- Domains: CXXC (10-13): Acts as a disulfide switch for the redox-sensitive transcriptional regulation of genes that function in thiol homeostasis.
- Modification: Cysteine oxidation of the CXXC motif
- Cofactor(s):
- Effectors of protein activity:
- Interactions: Spx-RpoA (C-terminal domain)
- Localization:
Database entries
- Structure:
- Swiss prot entry:
- KEGG entry:
- E.C. number:
Additional information
Expression and regulation
two promoters upstream of spx: SigA, SigW
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
Labs working on this gene/protein
Your additional remarks
References
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
