Difference between revisions of "NupN"
| Line 1: | Line 1: | ||
| − | * '''Description:''' | + | * '''Description:''' lipoprotein, part of guanosine transporter <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 10: | Line 10: | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || lipoprotein, part of guanosine transporter |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || uptake of guanosine |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 4.82 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 4.82 | ||
| Line 33: | Line 33: | ||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
{{SubtiWiki category|[[membrane proteins]]}}, | {{SubtiWiki category|[[membrane proteins]]}}, | ||
| − | {{SubtiWiki category|[[ | + | {{SubtiWiki category|[[biosynthesis/ acquisition of nucleotides]]}} |
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| Line 45: | Line 45: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * a ''[[nupN]] [[nupG]]'' double mutant is unable to utilize externally provided guanosine {{PubMed|21926227}} | ||
=== Database entries === | === Database entries === | ||
| Line 53: | Line 54: | ||
=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
| Line 96: | Line 95: | ||
=Expression and regulation= | =Expression and regulation= | ||
| + | * '''Operon:''' ''[[nupN]]-[[nupO]]-[[nupP]]-[[nupQ]]'' {{PubMed|21926227}} | ||
| − | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|21926227}} | |
| − | |||
| − | * '''[[Sigma factor]]:''' | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| − | ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) {{PubMed|12618455,21699902}} | + | ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) {{PubMed|12618455,21699902,21926227}} |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | ** [[CodY]]: transcription repression {{PubMed|12618455,21699902}} | + | ** [[CodY]]: transcription repression {{PubMed|12618455,21699902,21926227}} |
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 129: | Line 127: | ||
=References= | =References= | ||
| − | <pubmed>12618455,18763711, 20935095 21699902 </pubmed> | + | <pubmed>12618455,18763711, 20935095 21699902 21926227</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:39, 20 September 2011
- Description: lipoprotein, part of guanosine transporter
| Gene name | yufN |
| Synonyms | |
| Essential | no |
| Product | lipoprotein, part of guanosine transporter |
| Function | uptake of guanosine |
| MW, pI | 37 kDa, 4.82 |
| Gene length, protein length | 1050 bp, 350 aa |
| Immediate neighbours | malR, yufO |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
membrane proteins, biosynthesis/ acquisition of nucleotides
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU31540
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: BMP lipoprotein family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cell membrane (according to Swiss-Prot), membrane associated PubMed
Database entries
- Structure:
- UniProt: O05252
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Boris R Belitsky, Abraham L Sonenshein
CodY-mediated regulation of guanosine uptake in Bacillus subtilis.
J Bacteriol: 2011, 193(22);6276-87
[PubMed:21926227]
[WorldCat.org]
[DOI]
(I p)
Boris R Belitsky, Abraham L Sonenshein
Roadblock repression of transcription by Bacillus subtilis CodY.
J Mol Biol: 2011, 411(4);729-43
[PubMed:21699902]
[WorldCat.org]
[DOI]
(I p)
Shaun R Brinsmade, Roelco J Kleijn, Uwe Sauer, Abraham L Sonenshein
Regulation of CodY activity through modulation of intracellular branched-chain amino acid pools.
J Bacteriol: 2010, 192(24);6357-68
[PubMed:20935095]
[WorldCat.org]
[DOI]
(I p)
Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711]
[WorldCat.org]
[DOI]
(I p)
Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455]
[WorldCat.org]
[DOI]
(P p)
