Difference between revisions of "PutB"

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* '''Operon:'''  ''[[putB]]-[[putC]]-[[putP]]'' {{PubMed|21840319}}  
 
* '''Operon:'''  ''[[putB]]-[[putC]]-[[putP]]'' {{PubMed|21840319}}  
  
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|21840319}}  
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|21840319,21964733}}  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
** [[PutR]]: transcription activation {{PubMed|21840319}}  
+
** [[PutR]]: transcription activation {{PubMed|21840319,21964733}}  
 
** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
 
** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
 
** [[CodY]]: transcription repression, displacement of [[PutR]] {{PubMed|21840319}}  
 
** [[CodY]]: transcription repression, displacement of [[PutR]] {{PubMed|21840319}}  
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=References=
 
=References=
 
+
'''Additional publications:''' {{PubMed|21964733}}
 
<pubmed>14976255,21840319,14651647,12618455, </pubmed>
 
<pubmed>14976255,21840319,14651647,12618455, </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 09:28, 6 October 2011

  • Description: proline oxidase (L-proline, NAD)

Gene name putB
Synonyms ycgM
Essential no
Product proline oxidase (L-proline, NAD)
Function proline utilization
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 34 kDa, 7.291
Gene length, protein length 909 bp, 303 aa
Immediate neighbours ycgL, putC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YcgM context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

CodY regulon, PutR regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU03200

Phenotypes of a mutant

  • no growth with proline as single source of carbon or nitrogen PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor (according to Swiss-Prot)
  • Protein family: proline dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s): FadM

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced in the presence of proline (PutR) PubMed
    • expressed under conditions that trigger sporulation (Spo0A) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed