AnsA

• Description: L-asparaginase
  
  

Gene name	ansA
Synonyms
Essential	no
Product	L-asparaginase
Function	asparagine degradation
Metabolic function and regulation of this protein in SubtiPathways: Asp, Asn
MW, pI	36 kDa, 4.455
Gene length, protein length	987 bp, 329 aa
Immediate neighbours	ansB, ansR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

AnsR regulon

The gene

Basic information

• Locus tag: BSU23580

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-asparagine + H₂O = L-aspartate + NH₃ (according to Swiss-Prot)

• Protein family: asparaginase 1 family (according to Swiss-Prot)

• Paralogous protein(s): AnsZ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P26900

• KEGG entry: [3]

• E.C. number: 3.5.1.1

Expression and regulation

• Operon: ansA-ansB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the presence of asparagine (AnsR) PubMed

• Regulatory mechanism:
  • AnsR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP1153 (del ansAB::ermC) available in the Stülke lab

• Expression vector:

• lacZ fusion: pGP872 (in pAC5), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Susan H Fisher, Lewis V Wray
Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase.
J Bacteriol: 2002, 184(8);2148-54
[PubMed:11914346] [WorldCat.org] [DOI] (P p)

D Sun, P Setlow
Cloning and nucleotide sequence of the Bacillus subtilis ansR gene, which encodes a repressor of the ans operon coding for L-asparaginase and L-aspartase.
J Bacteriol: 1993, 175(9);2501-6
[PubMed:8478318] [WorldCat.org] [DOI] (P p)

D X Sun, P Setlow
Cloning, nucleotide sequence, and expression of the Bacillus subtilis ans operon, which codes for L-asparaginase and L-aspartase.
J Bacteriol: 1991, 173(12);3831-45
[PubMed:1711029] [WorldCat.org] [DOI] (P p)