Difference between revisions of "SubtInteract"

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(Protein complexes in B. subtilis)
(Protein complexes in B. subtilis)
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=== [[General stress proteins (controlled by SigB)|general stress response]]: the [[stressosome]]===
 
=== [[General stress proteins (controlled by SigB)|general stress response]]: the [[stressosome]]===
 
=== [[cell division]]: the [[divisome]]===
 
=== [[cell division]]: the [[divisome]]===
===DNA uptake: the [[pseudopilus]] {{PubMed|21278288}}===
+
===DNA uptake: the [[pseudopilus]] {{PubMed|21278288,16751195}}===
 
=== [[metabolism]]: the metabolons of glycolysis and the TCA cycle {{PubMed|19193632,20933603}}===
 
=== [[metabolism]]: the metabolons of glycolysis and the TCA cycle {{PubMed|19193632,20933603}}===
  
 
==Important publications==
 
==Important publications==
 
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 18366733 10694888 2200717 2441660  20969605 15451506 </pubmed>
 
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 18366733 10694888 2200717 2441660  20969605 15451506 </pubmed>

Revision as of 14:20, 8 April 2011

Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.

Methods to detect protein-protein interactions

  • Yeast Two Hybrid System PubMed
  • SPINE: A method to detect in vivo protein-protein interactions after cross-linking PubMed
  • in vivo detection of protein-protein interaction using DivIVA and GFP PubMed

Attention: Each technique detects only about 33% of all interactions PubMed

Visualization of protein-protein interactions in B. subtilis

Protein complexes in B. subtilis

DNA replication: the replisome

transcription: RNA polymerase

translation: the ribosome

RNA processing and degradation: the RNA degradosome

general stress response: the stressosome

cell division: the divisome

DNA uptake: the pseudopilus PubMed

metabolism: the metabolons of glycolysis and the TCA cycle PubMed

Important publications