LcfB

• Description: long-chain fatty-acid-CoA ligase, involved in surfactin production
  
  

Gene name	lcfB
Synonyms	yhfL
Essential	no
Product	long-chain fatty-acid-CoA ligase
Function	fatty acid degradation
Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation
MW, pI	56 kDa, 5.301
Gene length, protein length	1539 bp, 513 aa
Immediate neighbours	yhfK, yhfM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of lipids

This gene is a member of the following regulons

CcpA regulon, FadR regulon

The gene

Basic information

• Locus tag: BSU10270

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis PubMed

• Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

• Paralogous protein(s): LcfA, YhfT, YngI

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O07610

• KEGG entry: [3]

• E.C. number: 6.2.1.3

Expression and regulation

• Operon: lcfB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the presence of long-chain fatty acids (FadR) PubMed
  • subject to carbon catabolite repression (CcpA-HPr(Ser-P) PubMed

• Regulatory mechanism:
  • FadR: transcription repression PubMed
  • CcpA-HPr(Ser-P): transcription repression PubMed

• Additional information:

Biological materials

• Mutant: available in Mohamed Marahiel's lab PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Femke I Kraas, Verena Helmetag, Melanie Wittmann, Matthias Strieker, Mohamed A Marahiel
Functional dissection of surfactin synthetase initiation module reveals insights into the mechanism of lipoinitiation.
Chem Biol: 2010, 17(8);872-80
[PubMed:20797616] [WorldCat.org] [DOI] (I p)

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)