BsrF

• Description: small RNA
  
  

Gene name	bsrF
Synonyms	SR2
Essential	no
Product	small RNA
Function	unknown
Gene length	bp
Immediate neighbours	yobO, csaA
Gene sequence (+200bp)
Genetic context File:GenE context.gif

Categories containing this gene/protein

ncRNA

The gene

Basic information

• Locus tag:

Database entries

• BsubCyc: ?

• DBTBS entry: no entry

• SubtiList entry: no entry

• Structure:

Expression and regulation

• Operon: bsrF PubMed

• Sigma factor: SigA PubMed

• Regulation: weakly activated by CodY PubMed

• Regulatory mechanism: CodY: transcription activation PubMed

• Additional information:

Biological materials

• Mutants: DB104::Delta bsrF::cat, DB104::Delta bsrF::ery, 168::Delta bsrF::cat, 168::Delta bsrF::ery available in the Sabine Brantl lab

• Expression vectors:
  • pWSR2, multi-copy vector for tetracycline-inducible expression of bsrF (kanamycin resistance gene), available in the Sabine Brantl lab
  • pUCB2SR2, multi-copy vector for constitutive expression of bsrF under its own promoter (kanamycin resistance gene), available in the Sabine Brantl lab

• lacZ fusions: pACG1 to pACG19 containing regions of different size upstream of the bsrF promoter available in the Sabine Brantl lab

Labs working on this gene/RNA

• Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage
• Kouji Nakamura, Tsukuba, University, Japan

References

Heike Preis, Rita A Eckart, Rajani K Gudipati, Nadja Heidrich, Sabine Brantl
CodY activates transcription of a small RNA in Bacillus subtilis.
J Bacteriol: 2009, 191(17);5446-57
[PubMed:19542274] [WorldCat.org] [DOI] (I p)

Shinichi Saito, Hiroshi Kakeshita, Kouji Nakamura
Novel small RNA-encoding genes in the intergenic regions of Bacillus subtilis.
Gene: 2009, 428(1-2);2-8
[PubMed:18948176] [WorldCat.org] [DOI] (P p)