Difference between revisions of "SmpB"

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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
+
* impaired growth at high and low temperatures {{PubMed|15805528}}
 
=== Database entries ===
 
=== Database entries ===
  
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=== Additional information===
 
=== Additional information===
 
 
  
  
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:'''
+
* '''Interactions:''' [[SmpB]]-[[SsrA]]
  
 
* '''Localization:''' cytoplasm (according to Swiss-Prot)
 
* '''Localization:''' cytoplasm (according to Swiss-Prot)
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* '''Operon:'''  
 
* '''Operon:'''  
 +
** ''[[secG]]-[[yvaK]]-[[rnr]]-[[smpB]]-[[ssrA]]'' {{PubMed|17369301}}
 +
** ''[[yvaK]]-[[rnr]]-[[smpB]]-[[ssrA]]'' {{PubMed|17369301}}
 +
** ''[[smpB]]-[[ssrA]]'' {{PubMed|17369301}}
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** ''[[yvaK]]'': [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
  
* '''Regulatory mechanism:'''  
+
* '''Regulation:'''
 +
** ''[[yvaK]]'': induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
  
 
* '''Additional information:'''  
 
* '''Additional information:'''  
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=References=
 
=References=
  
<pubmed>18977770,11395451,17369301,12730326,,10881189, </pubmed>
+
<pubmed>18977770,11395451,17369301,12730326,,10881189, 15805528 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 07:36, 3 February 2011

  • Description: tmRNA-binding protein

Gene name smpB
Synonyms yvaI
Essential no
Product tmRNA-binding protein
Function trans-translation
MW, pI 17 kDa, 10.421
Gene length, protein length 468 bp, 156 aa
Immediate neighbours ssrA, rnr
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SmpB context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU33600

Phenotypes of a mutant

  • impaired growth at high and low temperatures PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: smpB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hiromi Ujiie, Tomoko Matsutani, Hisashi Tomatsu, Ai Fujihara, Chisato Ushida, Yasuhiko Miwa, Yasutaro Fujita, Hyouta Himeno, Akira Muto
Trans-translation is involved in the CcpA-dependent tagging and degradation of TreP in Bacillus subtilis.
J Biochem: 2009, 145(1);59-66
[PubMed:18977770] [WorldCat.org] [DOI] (I p)

Ji-Hyun Shin, Chester W Price
The SsrA-SmpB ribosome rescue system is important for growth of Bacillus subtilis at low and high temperatures.
J Bacteriol: 2007, 189(10);3729-37
[PubMed:17369301] [WorldCat.org] [DOI] (P p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Jeffrey H Withey, David I Friedman
A salvage pathway for protein structures: tmRNA and trans-translation.
Annu Rev Microbiol: 2003, 57;101-23
[PubMed:12730326] [WorldCat.org] [DOI] (P p)

T Wiegert, W Schumann
SsrA-mediated tagging in Bacillus subtilis.
J Bacteriol: 2001, 183(13);3885-9
[PubMed:11395451] [WorldCat.org] [DOI] (P p)

A W Karzai, E D Roche, R T Sauer
The SsrA-SmpB system for protein tagging, directed degradation and ribosome rescue.
Nat Struct Biol: 2000, 7(6);449-55
[PubMed:10881189] [WorldCat.org] [DOI] (P p)