CymR

• Description: pleiotropic regulator of sulphur metabolism
  
  

Gene name	cymR
Synonyms	yrzC
Essential	no
Product	transcription repressor
Function	regulation of sulphur metabolism
Metabolic function and regulation of this protein in SubtiPathways: Riboflavin / FAD, Cys, Met & Sulfate assimilation
MW, pI	11 kDa, 9.879
Gene length, protein length	333 bp, 111 aa
Immediate neighbours	yrvO, yrvN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

sulfur metabolism, regulators of core metabolism

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU27520

Phenotypes of a mutant

• impaired growth in the presence of cystine and increased sensitivity to hydrogen peroxide-, disulfide-, paraquat- and tellurite-induced stresses PubMed
• increased intracellular cysteine pool and hydrogen sulfide formation PubMed
• depletion of branched-chain amino acids PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: Rrf2 family of transcription regulators

• Paralogous protein(s):

Genes repressed by CymR

• ytmI-tcyJ-tcyK-tcyL-tcyM-tcyN-ytmO-ytnI-ytnJ-ribR-ytnL-ytnM PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): CysK acts as corepressor PubMed

• Effectors of protein activity:

• Interactions: CymR-CysK, the complex is formed in the presence of cysteine, and results in DNA binding and repression of genes of cysteine biosynthesis PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O34527

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cymR-yrvO-trmU PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Isabelle Martin-Verstraete, Institute Pasteur, Paris, France

References

The CymR regulon

Sergine Even, Pierre Burguière, Sandrine Auger, Olga Soutourina, Antoine Danchin, Isabelle Martin-Verstraete
Global control of cysteine metabolism by CymR in Bacillus subtilis.
J Bacteriol: 2006, 188(6);2184-97
[PubMed:16513748] [WorldCat.org] [DOI] (P p)