Difference between revisions of "LcfB"
| Line 39: | Line 39: | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| − | {{SubtiWiki regulon|[[FadR regulon]]}} | + | {{SubtiWiki regulon|[[CcpA regulon]]}}, {{SubtiWiki regulon|[[FadR regulon]]}} |
=The gene= | =The gene= | ||
| Line 106: | Line 106: | ||
* '''Regulation:''' | * '''Regulation:''' | ||
** induced in the presence of long-chain fatty acids ([[FadR]]) {{PubMed|17189250}} | ** induced in the presence of long-chain fatty acids ([[FadR]]) {{PubMed|17189250}} | ||
| + | ** subject to carbon catabolite repression ([[CcpA]]-[[PtsH|HPr(Ser-P)]] {{PubMed|21398533}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
** [[FadR]]: transcription repression {{PubMed|17189250}} | ** [[FadR]]: transcription repression {{PubMed|17189250}} | ||
| + | ** [[CcpA]]-[[PtsH|HPr(Ser-P)]]: transcription repression {{PubMed|21398533}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 131: | Line 133: | ||
=References= | =References= | ||
| − | + | '''Additional publications:''' {{PubMed|21398533}} | |
<pubmed>17189250,17919287 20797616</pubmed> | <pubmed>17189250,17919287 20797616</pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 12:57, 19 March 2011
- Description: long-chain fatty-acid-CoA ligase, involved in surfactin production
| Gene name | lcfB |
| Synonyms | yhfL |
| Essential | no |
| Product | long-chain fatty-acid-CoA ligase |
| Function | fatty acid degradation |
| Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation | |
| MW, pI | 56 kDa, 5.301 |
| Gene length, protein length | 1539 bp, 513 aa |
| Immediate neighbours | yhfK, yhfM |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU10270
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis PubMed
- Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: O07610
- KEGG entry: [3]
- E.C. number: 6.2.1.3
Additional information
Expression and regulation
- Operon: lcfB PubMed
- Regulation:
- induced in the presence of long-chain fatty acids (FadR) PubMed
- subject to carbon catabolite repression (CcpA-HPr(Ser-P) PubMed
- Regulatory mechanism:
- FadR: transcription repression PubMed
- CcpA-HPr(Ser-P): transcription repression PubMed
- Additional information:
Biological materials
- Mutant: available in Mohamed Marahiel's lab PubMed
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
