Difference between revisions of "NrdE"
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<br/><br/><br/><br/><br/><br/> | <br/><br/><br/><br/><br/><br/> | ||
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| + | = [[Categories]] containing this gene/protein = | ||
| + | {{SubtiWiki category|[[biosynthesis/ acquisition of nucleotides]]}}, | ||
| + | {{SubtiWiki category|[[essential genes]]}} | ||
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| + | = This gene is a member of the following [[regulons]] = | ||
| + | {{SubtiWiki regulon|[[ResD regulon]]}} | ||
=The gene= | =The gene= | ||
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=The protein= | =The protein= | ||
Revision as of 19:57, 8 December 2010
- Description: ribonucleoside-diphosphate reductase (major subunit), control of DNA/cell mass ratio
| Gene name | nrdE |
| Synonyms | |
| Essential | yes PubMed |
| Product | ribonucleoside-diphosphate reductase (major subunit) |
| Function | synthesis of deoxyribonucleoside triphosphates |
| Metabolic function and regulation of this protein in SubtiPathways: Purine salvage, Nucleotides (regulation) | |
| MW, pI | 80 kDa, 5.931 |
| Gene length, protein length | 2100 bp, 700 aa |
| Immediate neighbours | nrdI, nrdF |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
biosynthesis/ acquisition of nucleotides, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU17380
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin (according to Swiss-Prot)
- Protein family: ribonucleoside diphosphate reductase large chain family (according to Swiss-Prot)
- Paralogous protein(s): YosN
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- UniProt: P50620
- KEGG entry: [3]
- E.C. number:
Additional information
- subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and regulation
- Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Elisabeth Härtig, Braunschweig, Germany Homepage
Your additional remarks
References
Reviews
John Herrick, Bianca Sclavi
Ribonucleotide reductase and the regulation of DNA replication: an old story and an ancient heritage.
Mol Microbiol: 2007, 63(1);22-34
[PubMed:17229208]
[WorldCat.org]
[DOI]
(P p)
Original Publications
