Difference between revisions of "Easter egg"

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−
* '''Description:''' aminoglycoside 6-adenylyltransferase <br/><br/>
+
* '''Description:''' general stress protein, similar to DNA-3-methyladenine glycosidase II <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''aadK''
+
|''aag''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yxlJ ''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || aminoglycoside 6-adenylyltransferase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to streptomycin
+
|style="background:#ABCDEF;" align="center"|'''Function''' || survival of salt and ethanol stresses
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 4.755  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 21 kDa, 6.117  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 852 bp, 284 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 588 bp, 196 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yrdA]]'', ''[[yrpB]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxzF]]'', ''[[katX]]''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14620&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15888&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:aadK_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:yxlJ_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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__TOC__
 
__TOC__
  
−
<br/><br/>
+
<br/><br/><br/><br/>
  
 
=The gene=
 
=The gene=
Line 35: Line 35:
 
=== Basic information ===
 
=== Basic information ===
  
−
* '''Locus tag:''' BSU26790
+
* '''Locus tag:''' BSU38620
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' no entry
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yxlJ-yxzF.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11037]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12555]
  
 
=== Additional information===
 
=== Additional information===
Line 50: Line 50:
  
 
= Categories containing this gene/protein =
 
= Categories containing this gene/protein =
−
{{SubtiWiki category|[[resistance against toxins/ antibiotics]]}}
+
{{SubtiWiki category|[[DNA repair/ recombination/ based on similarity]]}},
 +
{{SubtiWiki category|[[general stress proteins (controlled by SigB)]]}}
 
=The protein=
 
=The protein=
  
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
−
* '''Catalyzed reaction/ biological activity:'''  
+
* '''Catalyzed reaction/ biological activity:''' removes hypoxanthine, 3-alkylated purines, and 1,N6-ethenoadenine from DNA; hypoxanthine and 1,N6-ethenoadenine are the preferred substrates [http://www.ncbi.nlm.nih.gov/sites/entrez/14729667 PubMed]
  
−
* '''Protein family:'''
+
* '''Protein family:''' DNA glycosylase MPG family (according to Swiss-Prot) AAG family of 3-methyladenine DNA glycosylases
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
Line 79: Line 80:
 
=== Database entries ===
 
=== Database entries ===
  
−
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2PBE 2PBE]
+
* '''Structure:'''
  
−
* '''UniProt:''' [http://www.uniprot.org/uniprot/P17585 P17585]
+
* '''UniProt:''' [http://www.uniprot.org/uniprot/P94378 P94378]
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU26790]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU38620]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 90: Line 91:
  
 
=Expression and regulation=
 
=Expression and regulation=
 +
* '''Operon:''' ''[[aag]]-[[yxzF]]'' {{PubMed|8990303}}
  
−
* '''Operon:'''  
+
* '''[[Sigma factor]]:''' [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
  
−
* '''[[Sigma factor]]:'''  
+
* '''Regulation:'''
−
 
+
** induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
−
* '''Regulation:'''
 
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
−
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =
Line 121: Line 122:
 
=References=
 
=References=
  
−
<pubmed>2550327,8293959,, </pubmed>
+
<pubmed>15805528 14729667, </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 14:22, 30 November 2010

  • Description: general stress protein, similar to DNA-3-methyladenine glycosidase II

Gene name aag
Synonyms yxlJ
Essential no
Product unknown
Function survival of salt and ethanol stresses
MW, pI 21 kDa, 6.117
Gene length, protein length 588 bp, 196 aa
Immediate neighbours yxzF, katX
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YxlJ context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Locus tag: BSU38620

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

Categories containing this gene/protein

DNA repair/ recombination/ based on similarity, general stress proteins (controlled by SigB)

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: removes hypoxanthine, 3-alkylated purines, and 1,N6-ethenoadenine from DNA; hypoxanthine and 1,N6-ethenoadenine are the preferred substrates PubMed
  • Protein family: DNA glycosylase MPG family (according to Swiss-Prot) AAG family of 3-methyladenine DNA glycosylases
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Randi M Aamodt, Pål Ø Falnes, Rune F Johansen, Erling Seeberg, Magnar Bjørås
The Bacillus subtilis counterpart of the mammalian 3-methyladenine DNA glycosylase has hypoxanthine and 1,N6-ethenoadenine as preferred substrates.
J Biol Chem: 2004, 279(14);13601-6
[PubMed:14729667] [WorldCat.org] [DOI] (P p)

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