Difference between revisions of "PGP1460"
| Line 11: | Line 11: | ||
*'''M13_puc_rev:''' 5‘-GGAAACAGCTATGACCATG-3‘ | *'''M13_puc_rev:''' 5‘-GGAAACAGCTATGACCATG-3‘ | ||
| + | Similar plasmid: [[pGP1459]] (for N-terminal Strep-tags) | ||
<pubmed> </pubmed> | <pubmed> </pubmed> | ||
Revision as of 07:37, 9 May 2012
The vector was constructed in the Stülke lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in B. subtilis. The plasmid confers resistance to ampicillin and kanamycin in E. coli and B. subtilis, respectively. To transform B. subtilis, the plasmid has to be linearized with ScaI. Just like pGP382 it can be used for the SPINE method.
Detailed information about the multiple cloning site can be found here: pGP382.
Genes cloned into this vector require a Shine-Dalgarno sequence.
Sequencing primers:
- M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
- M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘
Similar plasmid: pGP1459 (for N-terminal Strep-tags)
