Difference between revisions of "MreC"
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
− | ''mreC'' is essential under normal conditions {{PubMed|12682299}}. Depletion of MreC leads to a progressive increase in the width and a decrease in the length of the cell. This shape defect is consistent with a role for ''mreC'' in cell wall synthesis during elongation and has a similar phenotype to other genes with roles in elongation like ''[[rodA]]'' and the redundant gene pair ''[[pbpA]]'' and ''[[pbpH]]''. Electron microscopy of cells depleted of MreC shows regions of the cell where a thick and irregular cell wall has accumulated {{PubMed|12867458,16101995}}. ''mreC'' can be deleted provided that 0.5 M sucrose and 20 mM Mg(2+) is provided in the media, ''mreC'' is therefore conditionally essentail. The phenotype of the ''mreC'' deletion in these conditions is one characterised by extreamly fat and bloated cells that tend to grow in clusters {{PubMed|16101995}}. | + | * ''mreC'' is essential under normal conditions {{PubMed|12682299}}. |
+ | * Depletion of MreC leads to a progressive increase in the width and a decrease in the length of the cell. This shape defect is consistent with a role for ''mreC'' in cell wall synthesis during elongation and has a similar phenotype to other genes with roles in elongation like ''[[rodA]]'' and the redundant gene pair ''[[pbpA]]'' and ''[[pbpH]]''. | ||
+ | ** Electron microscopy of cells depleted of MreC shows regions of the cell where a thick and irregular cell wall has accumulated {{PubMed|12867458,16101995}}. | ||
+ | ** ''mreC'' can be deleted provided that 0.5 M sucrose and 20 mM Mg(2+) is provided in the media, ''mreC'' is therefore conditionally essentail. The phenotype of the ''mreC'' deletion in these conditions is one characterised by extreamly fat and bloated cells that tend to grow in clusters {{PubMed|16101995}}. | ||
=== Database entries === | === Database entries === | ||
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* '''Interactions:''' [[MreC]]-[[MreD]], interacts also with a subset of the [[penicillin-binding protein|PBPs]] [http://www.ncbi.nlm.nih.gov/pubmed/17427287 PubMed] | * '''Interactions:''' [[MreC]]-[[MreD]], interacts also with a subset of the [[penicillin-binding protein|PBPs]] [http://www.ncbi.nlm.nih.gov/pubmed/17427287 PubMed] | ||
− | * '''Localization:''' | + | * '''Localization:''' |
+ | ** GFP-MreC localises to the cell membrane in a helical pattern [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed]. | ||
+ | ** formation of helical clusters depends on the proton motive force {{PubMed|20566861}} | ||
=== Database entries === | === Database entries === | ||
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=References= | =References= | ||
− | + | ==Localization== | |
+ | <pubmed> 20566861 </pubmed> | ||
+ | ==Other original publications== | ||
<pubmed>18784364,16101995,1400224,18156271,17427287,,12867458 16101995 15612918 17993535 16344480 17427287 16344481 16484180 10954092 19643765 8459776 20623345 </pubmed> | <pubmed>18784364,16101995,1400224,18156271,17427287,,12867458 16101995 15612918 17993535 16344480 17427287 16344481 16484180 10954092 19643765 8459776 20623345 </pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 13:05, 19 July 2010
- Description: MreC is a cell shape determining protein and is associated with the MreB cytoskeleton in B. subtilis and other rod shaped bacteria.
Gene name | mreC |
Synonyms | |
Essential | yes PubMed |
Product | cell-shape determining protein |
Function | cell-shape determation |
MW, pI | 32 kDa, 6.248 |
Gene length, protein length | 870 bp, 290 aa |
Immediate neighbours | mreD, mreB |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU28020
Phenotypes of a mutant
- mreC is essential under normal conditions PubMed.
- Depletion of MreC leads to a progressive increase in the width and a decrease in the length of the cell. This shape defect is consistent with a role for mreC in cell wall synthesis during elongation and has a similar phenotype to other genes with roles in elongation like rodA and the redundant gene pair pbpA and pbpH.
- Electron microscopy of cells depleted of MreC shows regions of the cell where a thick and irregular cell wall has accumulated PubMed.
- mreC can be deleted provided that 0.5 M sucrose and 20 mM Mg(2+) is provided in the media, mreC is therefore conditionally essentail. The phenotype of the mreC deletion in these conditions is one characterised by extreamly fat and bloated cells that tend to grow in clusters PubMed.
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
Function
MreC functions in cell wall synthesis by, together with the MreB cytoskeleton, localizing the cell wall synthetic machinery to the correct part of the cell. MreC therefore ensures that the cell wall is made in the correct way to maintain the proper shape of the cell.
MreC in other organisms
MreC has been studied in other organisms where it has been shown to be important in cell shape determination.
- Escherichia coli PubMed PubMed
- Caulobacter cresentus PubMed PubMed
- Rhodobacter spheroides PubMed
- Streptomyces coelicolor PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: None/ structural protein
- Protein family: mreC family (according to Swiss-Prot) COG1793
- Paralogous protein(s): None
Extended information on the protein
- Kinetic information:
- Domains: Intracellular N-terminus, transmembrane domain, Coiled coil domain and C-terminal beta-sheet domain.
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- UniProt: Q01466
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: A non-polar inframe deletion strain named 3481 and a xylose dependent conditional mutant named 3461 is avaliable from the Errington lab PubMed.
- Expression vector:
- lacZ fusion:
- GFP fusion: A functional N-terminal GFP fusion has been made where the fusion protein is the only copy of the gene in the cell: strain 3417 PubMed.
- two-hybrid system:
- Antibody: antisera raised in rabit is avaliable from the Errington lab.
Labs working on this gene/protein
Jeff Errington, Newcastle University, UK homepage
Peter Graumann, Freiburg University, Germany homepage
Your additional remarks
mreC is an abbreviation of murein region e, gene C
References
Localization
Henrik Strahl, Leendert W Hamoen
Membrane potential is important for bacterial cell division.
Proc Natl Acad Sci U S A: 2010, 107(27);12281-6
[PubMed:20566861]
[WorldCat.org]
[DOI]
(I p)
Other original publications