Difference between revisions of "RpsJ"

From SubtiWiki
Jump to: navigation, search
(Biological materials)
Line 107: Line 107:
 
* '''Expression vector:'''
 
* '''Expression vector:'''
 
          
 
          
* '''lacZ fusion:''' pGP605 (in [[pAC6]]), available  in [[Stülke]] lab
+
* '''lacZ fusion:'''  
 +
** pGP605 (in [[pAC6]]), available  in [[Stülke]] lab
 +
** pGP1448 (in [[pAC7]]), available  in [[Stülke]] lab
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''

Revision as of 07:20, 19 August 2010

Gene name rpsJ
Synonyms tetA
Essential yes PubMed
Product ribosomal protein S10 (BS13)
Function translation
MW, pI 11 kDa, 10.207
Gene length, protein length 306 bp, 102 aa
Immediate neighbours ybaC, rplC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RpsJ context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU01150

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
    • SigA two promoters, about 140bp and 200bp upstream of the start codon PubMed
  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Tom Coenye, Peter Vandamme
Organisation of the S10, spc and alpha ribosomal protein gene clusters in prokaryotic genomes.
FEMS Microbiol Lett: 2005, 242(1);117-26
[PubMed:15621428] [WorldCat.org] [DOI] (P p)

Original publications