Difference between revisions of "YabT"
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| − | * '''Description:''' similar to Ser/Thr kinase <br/><br/> | + | * '''Description:''' similar to [[protein kinases and phosphatases|Ser/Thr kinase]] <br/><br/> |
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* '''Catalyzed reaction/ biological activity:''' ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot) | * '''Catalyzed reaction/ biological activity:''' ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot) | ||
| − | * '''Protein family:''' protein kinase domain (according to Swiss-Prot) | + | * '''Protein family:''' [[protein kinases and phosphatases|protein kinase]] domain (according to Swiss-Prot) |
* '''Paralogous protein(s):''' | * '''Paralogous protein(s):''' | ||
Revision as of 20:54, 19 May 2010
- Description: similar to Ser/Thr kinase
| Gene name | yabT |
| Synonyms | |
| Essential | no |
| Product | unknown |
| Function | unknown |
| MW, pI | 37 kDa, 9.943 |
| Gene length, protein length | 1014 bp, 338 aa |
| Immediate neighbours | yabS, tilS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU00660
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
- Protein family: protein kinase domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P37562
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP577 (erm), available in Stülke lab
- Expression vector:
- purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP391, available in Stülke lab
- purification from E. coli with N-terminal Strep-tag, in pGP172: pGP823, available in Stülke lab
- purification from E. coli with N-terminal His-tag, in pWH844: pGP1408, available in Stülke lab
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325]
[WorldCat.org]
[DOI]
(P p)
