Difference between revisions of "BshB2"
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| − | * '''Description:''' N- | + | * '''Description:''' N-acetylglucosamin-malate deacetylase, involved in bacillithiol synthesis <br/><br/> |
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || N-acetylglucosamin-malate deacetylase |
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of bacillithiol |
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 4.511 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 4.511 | ||
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''[[ypjD]]-[[dapB]]-[[mgsA]]-[[bshB2]]-[[bshA]]-[[cca]]-[[birA]]'' {{PubMed|20308541}} |
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
Revision as of 10:16, 7 April 2010
- Description: N-acetylglucosamin-malate deacetylase, involved in bacillithiol synthesis
| Gene name | BshB2 |
| Synonyms | yojG |
| Essential | no |
| Product | N-acetylglucosamin-malate deacetylase |
| Function | biosynthesis of bacillithiol |
| MW, pI | 16 kDa, 4.511 |
| Gene length, protein length | 426 bp, 142 aa |
| Immediate neighbours | rsbRC, yojF |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU19460
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): BshB1
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: O31857
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: bshB2 null mutant and bshB1 / bshB2 double null mutant available in John Helmann lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541]
[WorldCat.org]
[DOI]
(I p)
