NrgB

• Description: nitrogen-regulated PII-like protein
  
  

Gene name	nrgB
Synonyms	glnK
Essential	no
Product	nitrogen-regulated PII-like protein
Function	regulation of ammonium uptake
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	12 kDa, 8.825
Gene length, protein length	348 bp, 116 aa
Immediate neighbours	nrgA, bcrC
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU36520

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, transcription factors and their control, membrane proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: P(II) protein family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: NrgA-NrgB PubMed, TnrA-NrgB PubMed

• Localization: cell membrane (according to Swiss-Prot), membrane

Database entries

• Structure: 2NUU (the complex with NrgA, from E. coli) PubMed

• UniProt: Q07428

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nrgA-nrgB PubMed

• Sigma factor: SigA PubMed

• Regulation: induced by ammonium limitation
  • induced by ammonium limitation (TnrA) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: GP259 (cat), GP255 (nrgAB, cat), both available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in Jörg Stülke lab

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage