CysL

• Description: transcriptional activator of the cysJ-cysI operon
  
  

Gene name	cysL
Synonyms	ywfK, ipa-89d
Essential	no
Product	transcriptional regulator (LysR family)
Function	regulation of cysteine biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	34 kDa, 6.963
Gene length, protein length	897 bp, 299 aa
Immediate neighbours	ywfL, pta
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU37650

Phenotypes of a mutant

• unable to grow with sulfate or sulfite as the only sulfur source PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

sulfur metabolism, transcription factors and their control

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: LysR family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P39647

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cysL PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by sulfate, sulfite, and thiosulfate (CysL) PubMed

• Regulatory mechanism:
  • CysL: auto-repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Isabelle Guillouard, Sandrine Auger, Marie-Françoise Hullo, Farid Chetouani, Antoine Danchin, Isabelle Martin-Verstraete
Identification of Bacillus subtilis CysL, a regulator of the cysJI operon, which encodes sulfite reductase.
J Bacteriol: 2002, 184(17);4681-9
[PubMed:12169591] [WorldCat.org] [DOI] (P p)