AsnB

• Description: asparagine synthase (glutamine-hydrolysing)
  
  

Gene name	asnB
Synonyms	asn
Essential	no
Product	asparagine synthase (glutamine-hydrolysing))
Function	biosynthesis of asparagine
Metabolic function and regulation of this protein in SubtiPathways: Asp, Asn
MW, pI	72 kDa, 5.615
Gene length, protein length	1896 bp, 632 aa
Immediate neighbours	ytnA, metK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU30540

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-aspartate + L-glutamine + H₂O = AMP + diphosphate + L-asparagine + L-glutamate (according to Swiss-Prot)

• Protein family: asparagine synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P54420

• KEGG entry: [3]

• E.C. number: 6.3.5.4

Expression and regulation

• Operon: asnB-ytnA PubMed

• Sigma factor:

• Regulation: constitutive

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

K Yoshida, Y Fujita, S D Ehrlich
Three asparagine synthetase genes of Bacillus subtilis.
J Bacteriol: 1999, 181(19);6081-91
[PubMed:10498721] [WorldCat.org] [DOI] (P p)