Difference between revisions of "Hag"

From SubtiWiki
Jump to: navigation, search
(References)
Line 99: Line 99:
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12730172 PubMed]
 
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12730172 PubMed]
 +
** [[CsrA]]: translation inhibition {{PubMed|17555441}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''

Revision as of 07:23, 12 December 2009

  • Description: flagellin protein

Gene name hag
Synonyms
Essential no
Product flagellin protein
Function movement and chemotaxis
MW, pI 32 kDa, 4.782
Gene length, protein length 912 bp, 304 aa
Immediate neighbours yvyC, csrA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Hag context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU35360

Phenotypes of a mutant

No swarming motility on B medium. PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: bacterial flagellin family (according to Swiss-Prot)
  • Paralogous protein(s): YvzB (C-terminal domain of Hag)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: secreted (according to Swiss-Prot), extracellular (no signal peptide) PubMed, membrane PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant: GP901 (aphA3), GP902 (tet), both available in Stülke lab
  • Expression vector:
  • lacZ fusion: pGP1035 (in pAC6), pGP755 (in pAC7), there is also a series of promoter deletion variants in pAC6 and pAC7, all available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References