YcbK

• Description: similar to efflux system
  
  

Gene name	ycbK
Synonyms
Essential	no
Product	unknown
Function	unknown
Metabolic function and regulation of this protein in SubtiPathways: Phe, Tyr, Trp
MW, pI	33 kDa, 9.572
Gene length, protein length	936 bp, 312 aa
Immediate neighbours	rtpA, ycbL
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU02540

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: DUF6 domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P42243

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: rtpA-ycbK PubMed

• Sigma factor:
  • rtpA: SigA PubMed
  • ycbK: SigB PubMed

• Regulation:
  • rtpA: induced by tryptophan limitation (T-box in the mRNA leader) PubMed
  • ycbK:
    • induced by tryptophan limitation (MtrB in the mRNA leader) PubMed
    • induced by stress (SigB) PubMed

• Regulatory mechanism:
  • T-box: transcription termination/ antitermination PubMed
  • MtrB: control of translation initiation (access of the ribosome to the Shine-Dalgarno sequence)PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Helen Yakhnin, Alexander V Yakhnin, Paul Babitzke
The trp RNA-binding attenuation protein (TRAP) of Bacillus subtilis regulates translation initiation of ycbK, a gene encoding a putative efflux protein, by blocking ribosome binding.
Mol Microbiol: 2006, 61(5);1252-66
[PubMed:16879415] [WorldCat.org] [DOI] (P p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

J P Sarsero, E Merino, C Yanofsky
A Bacillus subtilis operon containing genes of unknown function senses tRNATrp charging and regulates expression of the genes of tryptophan biosynthesis.
Proc Natl Acad Sci U S A: 2000, 97(6);2656-61
[PubMed:10706627] [WorldCat.org] [DOI] (P p)