Difference between revisions of "Mdh"
Line 93: | Line 93: | ||
=Expression and regulation= | =Expression and regulation= | ||
* '''Operon:''' | * '''Operon:''' | ||
− | **''[[citZ]]-[[icd]]-[[mdh]]'' | + | **''[[citZ]]-[[icd]]-[[mdh]]'' {{PubMed|8045899}} |
− | **''[[icd]]-[[mdh]]'' | + | **''[[icd]]-[[mdh]]'' {{PubMed|8045899}} |
− | * '''Sigma factor:''' [[SigA]] | + | * '''Sigma factor:''' |
+ | ** ''[[citZ]]'': [[SigA]] {{PubMed|8045899}} | ||
+ | ** ''[[icd]]'': [[SigA]] {{PubMed|8045899}} | ||
− | * '''Regulation:''' ''[[citZ]]'': catabolite repression ([[CcpA]]) | + | * '''Regulation:''' |
− | ** | + | ** ''[[citZ]]'': catabolite repression ([[CcpA]]) {{PubMed|12100558}} |
− | ''[[mdh]]'': constitutive | + | ** ''[[citZ]]'': repression by glucose + glutamate ([[CcpC]]) {{PubMed|12100558}} |
+ | ** ''[[mdh]]'': constitutive | ||
− | * '''Regulatory mechanism:''' [[CcpA]]: transcription repression, [[CcpC]]: transcription repression | + | * '''Regulatory mechanism:''' |
+ | ** [[CcpA]]: transcription repression, [[CcpC]]: transcription repression {{PubMed|12100558}} | ||
** [[CcpC]]: transcription repression (molecular inducer: citrate) [http://www.ncbi.nlm.nih.gov/sites/entrez/10656796 PubMed] | ** [[CcpC]]: transcription repression (molecular inducer: citrate) [http://www.ncbi.nlm.nih.gov/sites/entrez/10656796 PubMed] | ||
Line 129: | Line 133: | ||
=References= | =References= | ||
− | <pubmed>10656796,18763711 14284712 922015 </pubmed> | + | <pubmed>10656796,18763711 14284712 922015 12100558 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 20:57, 17 December 2009
- Description: malate dehydrogenase
Gene name | mdh |
Synonyms | citH |
Essential | no |
Product | malate dehydrogenase |
Function | TCA cycle |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Central C-metabolism | |
MW, pI | 33 kDa, 4.727 |
Gene length, protein length | 936 bp, 312 aa |
Immediate neighbours | phoP, icd |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU29120
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (S)-malate + NAD+ = oxaloacetate + NADH (according to Swiss-Prot)
- Protein family: MDH type 3 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Reversible Michaelis-Menten PubMed
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot), membrane associated PubMed
Database entries
- Structure: 1EMD (E.coli)
- UniProt: P49814
- KEGG entry: [3]
- E.C. number: 1.1.1.37
Additional information
The enzyme is a tetramer PubMed
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP719 (spc), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References