Difference between revisions of "BioI"
| Line 87: | Line 87: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | + | * '''Operon:''' ''[[bioW]]-[[bioA]]-[[bioF]]-[[bioD]]-[[bioB]]-[[bioI]]-[[ytbQ]]'' {{PubMed|8763940,8892842}} | |
| − | * '''Operon:''' | ||
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** repressed in the presence of biotin ([[BirA]]) {{PubMed|8763940,8892842}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[BirA]]: transcription repression {{PubMed|8763940,8892842}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 118: | Line 119: | ||
=References= | =References= | ||
| − | <pubmed>,8763940, | + | <pubmed>,8763940,8892842,15449931, 15449930 12538057</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 19:34, 19 December 2009
- Description: cytochrome P450 enzyme
| Gene name | bioI |
| Synonyms | |
| Essential | no |
| Product | cytochrome P450 enzyme |
| Function | biosynthesis of biotin |
| MW, pI | 44 kDa, 6.2 |
| Gene length, protein length | 1185 bp, 395 aa |
| Immediate neighbours | ytbQ, bioB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU30190
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: cytochrome P450 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure: 3EJE
- UniProt: P53554
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Rachel J Lawson, David Leys, Michael J Sutcliffe, Carol A Kemp, Myles R Cheesman, Susan J Smith, John Clarkson, W Ewen Smith, Ihtshamul Haq, John B Perkins, Andrew W Munro
Thermodynamic and biophysical characterization of cytochrome P450 BioI from Bacillus subtilis.
Biochemistry: 2004, 43(39);12410-26
[PubMed:15449931]
[WorldCat.org]
[DOI]
(P p)
Rachel J Lawson, Claes von Wachenfeldt, Ihtshamul Haq, John Perkins, Andrew W Munro
Expression and characterization of the two flavodoxin proteins of Bacillus subtilis, YkuN and YkuP: biophysical properties and interactions with cytochrome P450 BioI.
Biochemistry: 2004, 43(39);12390-409
[PubMed:15449930]
[WorldCat.org]
[DOI]
(P p)
Amanda J Green, Andrew W Munro, Myles R Cheesman, Graeme A Reid, Claes von Wachenfeldt, Stephen K Chapman
Expression, purification and characterisation of a Bacillus subtilis ferredoxin: a potential electron transfer donor to cytochrome P450 BioI.
J Inorg Biochem: 2003, 93(1-2);92-9
[PubMed:12538057]
[WorldCat.org]
[DOI]
(P p)
J B Perkins, S Bower, C L Howitt, R R Yocum, J Pero
Identification and characterization of transcripts from the biotin biosynthetic operon of Bacillus subtilis.
J Bacteriol: 1996, 178(21);6361-5
[PubMed:8892842]
[WorldCat.org]
[DOI]
(P p)
S Bower, J B Perkins, R R Yocum, C L Howitt, P Rahaim, J Pero
Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.
J Bacteriol: 1996, 178(14);4122-30
[PubMed:8763940]
[WorldCat.org]
[DOI]
(P p)
