Difference between revisions of "MetK"
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=Expression and regulation= | =Expression and regulation= | ||
− | * '''Operon:''' | + | * '''Operon:''' ''[[metK]]'' {{PubMed|9387221}} |
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
− | * '''Regulation:''' induced by methionine starvation ([[S-box]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10094622 PubMed] | + | * '''Regulation:''' |
+ | ** induced by methionine starvation ([[S-box]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10094622 PubMed] | ||
− | * '''Regulatory mechanism:''' [[S-box]]: | + | * '''Regulatory mechanism:''' |
+ | ** [[S-box]]: [[riboswitch]], binds S-adenosylmethionine resulting in transcription termination [http://www.ncbi.nlm.nih.gov/sites/entrez/10094622 PubMed] | ||
* '''Additional information:''' | * '''Additional information:''' | ||
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=References= | =References= | ||
− | <pubmed>10094622,16672621,, </pubmed> | + | <pubmed>10094622,16672621,9387221 , </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 20:18, 19 December 2009
- Description: S-adenosylmethionine synthetase
Gene name | metK |
Synonyms | metE |
Essential | yes PubMed |
Product | S-adenosylmethionine synthetase |
Function | S-adenosylmethionine synthesis |
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation | |
MW, pI | 43 kDa, 5.025 |
Gene length, protein length | 1200 bp, 400 aa |
Immediate neighbours | asnB, pckA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU30550
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine (according to Swiss-Prot)
- Protein family: AdoMet synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P54419
- KEGG entry: [3]
- E.C. number: 2.5.1.6
Additional information
Expression and regulation
- Regulatory mechanism:
- S-box: riboswitch, binds S-adenosylmethionine resulting in transcription termination PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References