Difference between revisions of "PGP172"
| Line 2: | Line 2: | ||
[[File:PGP172_cloning_region.jpeg|250px|thumb|right|'''pGP172 cloning region: click to enlarge''']] | [[File:PGP172_cloning_region.jpeg|250px|thumb|right|'''pGP172 cloning region: click to enlarge''']] | ||
| + | '''Applications:''' | ||
| − | + | Vector pGP172 was designed for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein. The ''E. coli'' strain BL21(DE3)/pLysS serves as a host strain and the expression of the desired protein is induced by the addition of 1 mM IPTG to the culture. pGP172 confers resistance to ampicillin in ''E. coli''. The plasmid was constructed in the [[Stülke]] lab and it is based on the vector pET3C. pGP172 is similar [[plasmid]] to [[pGP574]]. | |
| − | + | Sequencing primers: | |
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
*'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’ | *'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’ | ||
*'''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’ | *'''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’ | ||
<pubmed> 14717704 </pubmed> | <pubmed> 14717704 </pubmed> | ||
Revision as of 14:57, 25 February 2010
Applications:
Vector pGP172 was designed for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein. The E. coli strain BL21(DE3)/pLysS serves as a host strain and the expression of the desired protein is induced by the addition of 1 mM IPTG to the culture. pGP172 confers resistance to ampicillin in E. coli. The plasmid was constructed in the Stülke lab and it is based on the vector pET3C. pGP172 is similar plasmid to pGP574.
Sequencing primers:
- CD13: 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
- NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
Matthias Merzbacher, Christian Detsch, Wolfgang Hillen, Jörg Stülke
Mycoplasma pneumoniae HPr kinase/phosphorylase.
Eur J Biochem: 2004, 271(2);367-74
[PubMed:14717704]
[WorldCat.org]
[DOI]
(P p)
