Difference between revisions of "CotX"
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| − | * '''Description:''' spore coat protein (insoluble fraction) <br/><br/> | + | * '''Description:''' spore coat protein (insoluble fraction), necessary for the assembly of the spre crust, the outermost layer of the spore coat<br/><br/> |
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|style="background:#ABCDEF;" align="center"| '''Product''' || spore coat protein (insoluble fraction) | |style="background:#ABCDEF;" align="center"| '''Product''' || spore coat protein (insoluble fraction) | ||
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || spore coat assembly |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 18 kDa, 4.228 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 18 kDa, 4.228 | ||
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=References= | =References= | ||
| − | <pubmed>7519271,10788508,,12107147, </pubmed> | + | <pubmed>7519271,10788508, 20451384,12107147, </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:21, 11 May 2010
- Description: spore coat protein (insoluble fraction), necessary for the assembly of the spre crust, the outermost layer of the spore coat
| Gene name | cotX |
| Synonyms | |
| Essential | no |
| Product | spore coat protein (insoluble fraction) |
| Function | spore coat assembly |
| MW, pI | 18 kDa, 4.228 |
| Gene length, protein length | 516 bp, 172 aa |
| Immediate neighbours | cotY, cotW |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU11760
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: spore wall (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: Q08313
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
cotX PubMed
- Sigma factor: before cotV: SigK PubMed
- Regulation: repressed by casamino acids PubMed
- Regulatory mechanism: upstream of cotX: SpoIIID: repression PubMed, upstream of both promoters: GerE: transcription activation PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Peter T McKenney, Adam Driks, Haig A Eskandarian, Paul Grabowski, Jonathan Guberman, Katherine H Wang, Zemer Gitai, Patrick Eichenberger
A distance-weighted interaction map reveals a previously uncharacterized layer of the Bacillus subtilis spore coat.
Curr Biol: 2010, 20(10);934-8
[PubMed:20451384]
[WorldCat.org]
[DOI]
(I p)
Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147]
[WorldCat.org]
[DOI]
(P p)
H Ichikawa, L Kroos
Combined action of two transcription factors regulates genes encoding spore coat proteins of Bacillus subtilis.
J Biol Chem: 2000, 275(18);13849-55
[PubMed:10788508]
[WorldCat.org]
[DOI]
(P p)
J Zhang, H Ichikawa, R Halberg, L Kroos, A I Aronson
Regulation of the transcription of a cluster of Bacillus subtilis spore coat genes.
J Mol Biol: 1994, 240(5);405-15
[PubMed:7519271]
[WorldCat.org]
[DOI]
(P p)
