Difference between revisions of "CysI"
| Line 40: | Line 40: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * unable to grow with sulfate or sulfite as the only sulfur source {{PubMed|12169591}} | ||
=== Database entries === | === Database entries === | ||
| Line 89: | Line 90: | ||
=Expression and regulation= | =Expression and regulation= | ||
| + | * '''Operon:''' ''[[cysI]]-[[cysJ]]'' {{PubMed|12169591}} | ||
| − | * ''' | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|12169591}} |
| − | * ''' | + | * '''Regulation:''' |
| − | + | ** induced by sulfite, sulfate, and thiosulfate ([[CysL]]) {{PubMed|12169591}} | |
| − | * | + | ** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[CysL]]: transcription activation {{PubMed|12169591}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 08:18, 29 December 2009
- Description: sulfite reductase (NADPH2)
| Gene name | cysI |
| Synonyms | yvgR |
| Essential | no |
| Product | sulfite reductase (NADPH2) |
| Function | sulfite reduction |
| Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation | |
| MW, pI | 67 kDa, 4.573 |
| Gene length, protein length | 1815 bp, 605 aa |
| Immediate neighbours | cysJ, helD |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU33440
Phenotypes of a mutant
- unable to grow with sulfate or sulfite as the only sulfur source PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: O32213
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Isabelle Guillouard, Sandrine Auger, Marie-Françoise Hullo, Farid Chetouani, Antoine Danchin, Isabelle Martin-Verstraete
Identification of Bacillus subtilis CysL, a regulator of the cysJI operon, which encodes sulfite reductase.
J Bacteriol: 2002, 184(17);4681-9
[PubMed:12169591]
[WorldCat.org]
[DOI]
(P p)
Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147]
[WorldCat.org]
[DOI]
(P p)
J R van der Ploeg, M Barone, T Leisinger
Functional analysis of the Bacillus subtilis cysK and cysJI genes.
FEMS Microbiol Lett: 2001, 201(1);29-35
[PubMed:11445163]
[WorldCat.org]
[DOI]
(P p)
