SacA

• Description: phosphosucrase
  
  

Gene name	sacA
Synonyms	ipa-50d
Essential	no
Product	phosphosucrase
Function	utilization of sucrose
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	54 kDa, 5.34
Gene length, protein length	1440 bp, 480 aa
Immediate neighbours	ywdA, sacP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU38040

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 32 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P07819

• KEGG entry: [3]

• E.C. number: 3.2.1.26

Expression and regulation

• Operon: sacP-sacA-ywdA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by sucrose (SacT) PubMed
  • repressed by glucose (CcpA)

• Regulatory mechanism:
  • SacT: binding to a RNA switch results in transcription antitermination PubMed
  • CcpA: transcription repression

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

M Arnaud, M Débarbouillé, G Rapoport, M H Saier, J Reizer
In vitro reconstitution of transcriptional antitermination by the SacT and SacY proteins of Bacillus subtilis.
J Biol Chem: 1996, 271(31);18966-72
[PubMed:8702561] [WorldCat.org] [DOI] (P p)

M Debarbouille, M Arnaud, A Fouet, A Klier, G Rapoport
The sacT gene regulating the sacPA operon in Bacillus subtilis shares strong homology with transcriptional antiterminators.
J Bacteriol: 1990, 172(7);3966-73
[PubMed:2163394] [WorldCat.org] [DOI] (P p)

A Fouet, A Klier, G Rapoport
Nucleotide sequence of the sucrase gene of Bacillus subtilis.
Gene: 1986, 45(2);221-5
[PubMed:3100393] [WorldCat.org] [DOI] (P p)