Difference between revisions of "LeuS"
| Line 92: | Line 92: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''[[leuS]]'' {{PubMed|1317842}} |
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** induced by leucine limitation ([[T-box]]) {{PubMed|1317842}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[T-box]]: RNA switch, binding of uncharged tRNA-Leu results in transcription antitermination {{PubMed|1317842}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 121: | Line 123: | ||
=References= | =References= | ||
| − | + | ==Reviews== | |
<pubmed>19258532,, </pubmed> | <pubmed>19258532,, </pubmed> | ||
| + | ==Original Publications== | ||
| + | <pubmed>1317842,, </pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 19:52, 19 December 2009
- Description: Leu-tRNA synthetase
| Gene name | leuS |
| Synonyms | |
| Essential | yes PubMed |
| Product | leucyl-tRNA synthetase |
| Function | translation |
| Metabolic function and regulation of this protein in SubtiPathways: tRNA charging | |
| MW, pI | 91 kDa, 4.857 |
| Gene length, protein length | 2412 bp, 804 aa |
| Immediate neighbours | ytwF, ytvB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU30320
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu) (according to Swiss-Prot)
- Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P36430
- KEGG entry: [3]
- E.C. number: 6.1.1.4
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532]
[WorldCat.org]
[DOI]
(I p)
Original Publications
P B Vander Horn, S A Zahler
Cloning and nucleotide sequence of the leucyl-tRNA synthetase gene of Bacillus subtilis.
J Bacteriol: 1992, 174(12);3928-35
[PubMed:1317842]
[WorldCat.org]
[DOI]
(P p)
