Difference between revisions of "FadB"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
 
 
* '''Operon:'''  
 
* '''Operon:'''  
 +
** ''[[lcfA]]-[[fadR]]-[[fadB]]-[[etfB]]-[[etfA]]'' {{PubMed|17189250}}
 +
** ''[[fadR]]-[[fadB]]-[[etfB]]-[[etfA]]'' {{PubMed|17189250}}
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
 +
** ''[[fadR]]'': [[SigA]] {{PubMed|17189250}}
 +
 +
* '''Regulation:'''
 +
** induced by long-chain fatty acids ([[FadR]]) {{PubMed|17189250}}
 +
** repressed by glucose (3.8-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] 
 +
 +
* '''Regulatory mechanism:'''
 +
** [[FadR]]: transcription repression {{PubMed|17189250}}
  
* '''Regulation:''' repressed by glucose (3.8-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] 
+
* '''Regulation:'''  
 
** repressed in the absence of long-chain fatty acids ([[FadR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/17189250 PubMed]
 
** repressed in the absence of long-chain fatty acids ([[FadR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/17189250 PubMed]
 +
**  [[CcpA]]: transcription repression
  
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression
+
* '''Additional information:'''
** [[FadR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/17189250 PubMed]
 
 
 
* '''Additional information:'''  
 
  
 
=Biological materials =
 
=Biological materials =

Revision as of 21:26, 15 December 2009

  • Description: 3-hydroxyacyl-CoA dehydratase

Gene name fadB
Synonyms ysiB
Essential no
Product 3-hydroxyacyl-CoA dehydratase
Function fatty acid degradation
MW, pI 27 kDa, 4.951
Gene length, protein length 774 bp, 258 aa
Immediate neighbours etfB, fadR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YsiB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU28540

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O (according to Swiss-Prot)
  • Protein family: enoyl-CoA hydratase/isomerase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Regulation:
    • repressed in the absence of long-chain fatty acids (FadR) PubMed
    • CcpA: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)