Rny

• Description: Rny catalyzes the hydrolysis of both 2',3'-cyclic AMP and 2',3'-cyclic GMP into 3'-AMP and 3'-GMP and is a putative RNase
  
  

Gene name	rny
Synonyms	ymdA
Essential	yes
Product	2',3'-cyclic-nucleotide 2'-phosphodiesterase
Function	hydrolysis of AMP and GMP& putative RNase
MW, pI	58,7 kDa, 5.39
Gene length, protein length	1560 bp, 520 amino acids
Immediate neighbours	pdbX, ymdB
Gene sequence (+200bp)	Protein sequence
Genetic context File:GenE context.gif

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry:

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Nucleoside 2',3'-cyclic phosphate + H(2)O = nucleoside 3'-phosphate

• Protein family: 2',3' cyclic nucleotide phosphodiesterase family

• Paralogous protein(s): similar to unknown proteins

Extended information on the protein

• Kinetic information:

• Domains:
  • transmembrane domain (4–24)
  • KH domain (210–273)
  • HD domain (336–429)

• Modification:

• Cofactor(s): metal-dependent

• Effectors of protein activity:

• Interactions:

• Localization: Single-pass membrane protein

Database entries

• Structure:

• Swiss prot entry: [2]

• KEGG entry: [3]

• E.C. number: [4]

Additional information

required for processing of gapA operon mRNA

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Labs working on this gene/protein

Jörg Stülke, University of Göttingen, Germany Homepage

References

1. Commichau, F. M., Rothe, F. M., Herzberg, C., Wagner, E., Hellwig, D., Lehnik-Habrink, M., Hammer, E., Völker, U. & Stülke, J. Novel activities of glycolytic enzymes in Bacillus subtilis: Interactions with essential proteins involved in mRNA processing. subm.
2. Hunt, A., Rawlins, J. P., Thomaides, H. B., and Errington, J. (2006) Functional analysis of 11 putative essential genes in Bacillus subtilis. Microbiology 152, 2895-2907. PubMed