Difference between revisions of "Nos"
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''[[nos]]-[[yflK]]'' (according to [http://dbtbs.hgc.jp/COG/prom/yflMK.html DBTBS]) |
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
| Line 96: | Line 96: | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | * '''Additional information:''' | + | * '''Additional information:''' Note that the gene ''[[yflL]]'' is located between ''[[nos]]'' and ''[[yflK]]'', but on the opposite strand. |
=Biological materials = | =Biological materials = | ||
Revision as of 18:03, 31 August 2009
- Description: nitric-oxide synthase
| Gene name | nos |
| Synonyms | yflM |
| Essential | no |
| Product | nitric-oxide synthase |
| Function | production of nitric oxide |
| MW, pI | 38 kDa, 5.528 |
| Gene length, protein length | 1008 bp, 336 aa |
| Immediate neighbours | yflN, yflL |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU07630
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: Bacterial NOS oxygenase subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- UniProt: O34453
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information: Note that the gene yflL is located between nos and yflK, but on the opposite strand.
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Kartikeya Pant, Alexandrine M Bilwes, Subrata Adak, Dennis J Stuehr, Brian R Crane
Structure of a nitric oxide synthase heme protein from Bacillus subtilis.
Biochemistry: 2002, 41(37);11071-9
[PubMed:12220171]
[WorldCat.org]
[DOI]
(P p)
Subrata Adak, Kulwant S Aulak, Dennis J Stuehr
Direct evidence for nitric oxide production by a nitric-oxide synthase-like protein from Bacillus subtilis.
J Biol Chem: 2002, 277(18);16167-71
[PubMed:11856757]
[WorldCat.org]
[DOI]
(P p)
