Difference between revisions of "LigA"
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''[[pcrB]]-[[pcrA]]-[[ligA]]-[[yerH]]'' {{PubMed|9701819}} |
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| − | ** | + | ** induced by DNA damage ([[LexA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/16267290 PubMed] |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
** [[LexA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/16267290 PubMed] | ** [[LexA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/16267290 PubMed] | ||
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 19:35, 27 August 2009
- Description: DNA ligase (NAD-dependent)
| Gene name | ligA |
| Synonyms | lig, yerG |
| Essential | yes PubMed |
| Product | DNA ligase (NAD-dependent) |
| Function | replication |
| MW, pI | 74 kDa, 4.708 |
| Gene length, protein length | 2004 bp, 668 aa |
| Immediate neighbours | pcrA, yerH |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU06620
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m) (according to Swiss-Prot)
- Protein family: BRCT domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure: 1B04 (adenylation domain, Geobacillus stearothermophilus)
- UniProt: O31498
- KEGG entry: [3]
- E.C. number: 6.5.1.2
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
