PurH

• Description: phosphoribosylaminoimidazole carboxamide formyltransferase
  
  

Gene name	purH
Synonyms	purJ
Essential	no
Product	phosphoribosylaminoimidazole carboxamide formyltransferase and inosine-monophosphate cyclohydrolase
Function	purine biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: Purine synthesis, Nucleotides (regulation)
MW, pI	55 kDa, 5.133
Gene length, protein length	1536 bp, 512 aa
Immediate neighbours	purN, purD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU06520

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (according to Swiss-Prot)

• Protein family: purH family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1ZCZ (from Thermotoga maritima, 36% identity, 54% similarity) PubMed

• UniProt: P12048

• KEGG entry: [3]

• E.C. number: 2.1.2.3

Expression and regulation

• Operon: purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of purine of purine nucleotides (PurR) PubMed
  • repressed in the presence of adenine or adenosine(PurR) PubMed
  • repressed in the presence of guanine(G-box) PubMed

• Regulatory mechanism:
  • PurR: transcription repression PubMed
  • PurR: transcription repression (molecular inducer: PRPP) PubMed
  • G-box: transcription termination/ antitermination (riboswitch) PubMed1 PubMed2

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lars Engholm Johansen, Per Nygaard, Catharina Lassen, Yvonne Agersø, Hans H Saxild
Definition of a second Bacillus subtilis pur regulon comprising the pur and xpt-pbuX operons plus pbuG, nupG (yxjA), and pbuE (ydhL).
J Bacteriol: 2003, 185(17);5200-9
[PubMed:12923093] [WorldCat.org] [DOI] (P p)

M Weng, P L Nagy, H Zalkin
Identification of the Bacillus subtilis pur operon repressor.
Proc Natl Acad Sci U S A: 1995, 92(16);7455-9
[PubMed:7638212] [WorldCat.org] [DOI] (P p)

D J Ebbole, H Zalkin
Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis.
J Biol Chem: 1987, 262(17);8274-87
[PubMed:3036807] [WorldCat.org] (P p)