MmgE

• Description: putative methyl-cis-aconitase
  
  

Gene name	mmgE
Synonyms	yqiP, prpD
Essential	no
Product	unknown
Function	mother cell metabolism
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	52 kDa, 6.111
Gene length, protein length	1416 bp, 472 aa
Immediate neighbours	yqiQ, mmgD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU24130

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H₂O (according to Swiss-Prot)

• Protein family: prpD family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P45859

• KEGG entry: [3]

• E.C. number: 4.2.1.79

Expression and regulation

• Operon:

• Sigma factor:

• Regulation: repressed by glucose (CcpA)

• Regulatory mechanism: CcpA: transcription repression

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

E M Bryan, B W Beall, C P Moran
A sigma E dependent operon subject to catabolite repression during sporulation in Bacillus subtilis.
J Bacteriol: 1996, 178(16);4778-86
[PubMed:8759838] [WorldCat.org] [DOI] (P p)