PdaB

• Description: polysaccharide deacetylase
  
  

Gene name	pdaB
Synonyms	ybaN
Essential	no
Product	polysaccharide deacetylase
Function	spore cortex formation
MW, pI	28 kDa, 9.182
Gene length, protein length	762 bp, 254 aa
Immediate neighbours	kbaA, rrnI-16S
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU01570

Phenotypes of a mutant

pdaB ytrI and pdaB ytrH mutants are deficient in sporulation

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: polysaccharide deacetylase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: forespore (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P50865

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Tatsuya Fukushima, Taichi Tanabe, Hiroki Yamamoto, Shigeo Hosoya, Tsutomu Sato, Hirofumi Yoshikawa, Junichi Sekiguchi
Characterization of a polysaccharide deacetylase gene homologue (pdaB) on sporulation of Bacillus subtilis.
J Biochem: 2004, 136(3);283-91
[PubMed:15598884] [WorldCat.org] [DOI] (P p)

Jessica M Silvaggi, David L Popham, Adam Driks, Patrick Eichenberger, Richard Losick
Unmasking novel sporulation genes in Bacillus subtilis.
J Bacteriol: 2004, 186(23);8089-95
[PubMed:15547282] [WorldCat.org] [DOI] (P p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)