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| − | * '''Description:''' replication initiation protein <br/><br/>
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| − | {| align="right" border="1" cellpadding="2"
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| − | |-
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| − | |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| − | |''dnaA''
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| − | |-
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| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''dnaH, dnaJ, dnaK ''
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| − | |-
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| − | |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| − | |-
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || replication initiation protein
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| − | |-
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || DNA replication
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| − | |-
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| − | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 6.035
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| − | |-
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| − | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1338 bp, 446 aa
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| − | |-
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| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rpmH]]'', ''[[dnaN]]''
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| − | |-
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| − | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB11777]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| − | |-
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| − | |colspan="2" | '''Genetic context''' <br/> [[Image:DnaA_dnaN_yaaA_recF_yaaB_gyrB_context.png]]
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| − | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| − | |-
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| − | |}
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| − | __TOC__
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| − |
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| − | <br/><br/>
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| − |
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| − | =The gene=
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| − |
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| − | === Basic information ===
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| − | * '''Locus tag:''' BSU00010
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| − |
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| − | ===Phenotypes of a mutant ===
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| − | essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| − |
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| − | === Database entries ===
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| − | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/dnaAN.html]
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| − | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10065]
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| − |
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| − | === Additional information===
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| − |
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| − |
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| − | =The protein=
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| − |
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| − | === Basic information/ Evolution ===
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| − | * '''Catalyzed reaction/ biological activity:'''
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| − | * '''Protein family:''' dnaA family (according to Swiss-Prot)
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| − |
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| − | * '''Paralogous protein(s):'''
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| − |
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| − | === Extended information on the protein ===
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| − | * '''Kinetic information:'''
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| − | * '''Domains:'''
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| − | * '''Modification:'''
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| − | * '''Cofactor(s):'''
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| − | * '''Effectors of protein activity:'''
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| − | * '''Interactions:''' [[DnaA]]-[[YabA]], [[DnaA]]-[[YabA]]-[[DnaN]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12060778 PubMed], [[DnaA]]-[[DnaD]] [http://www.ncbi.nlm.nih.gov/sites/entrez/11222620 PubMed]
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| − | * '''Localization:''' cytoplasm (according to Swiss-Prot), throughout the cytoplasm [http://www.ncbi.nlm.nih.gov/sites/entrez/10844689 PubMed]
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| − |
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| | === Database entries === | | === Database entries === |
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| | * '''Structure:''' | | * '''Structure:''' |
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| − | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P05648 P05648] | + | * '''UniProt:''' [http://www.uniprot.org/uniprot/P05648 P05648] |
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| | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU00010] | | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU00010] |
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| | * '''E.C. number:''' | | * '''E.C. number:''' |
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| − | === Additional information===
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| − | =Expression and regulation=
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| − | * '''Operon:''' ''[[dnaA]]-[[dnaN]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed]
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| − | * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed]
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| − | * '''Regulation:''' negatively controlled by [[DnaA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/2168872 PubMed] and [[Spo0A]] [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| − | ** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| − | * '''Regulatory mechanism:'''
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| − | ** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
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| − | * '''Additional information:'''
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| − |
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| − | =Biological materials =
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| − | * '''Mutant:'''
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| − | * '''Expression vector:'''
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| − | * '''lacZ fusion:'''
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| − | * '''GFP fusion:'''
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| − | * '''two-hybrid system:'''
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| − | * '''Antibody:'''
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| − | =Labs working on this gene/protein=
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| − | [[Philippe Noirot]], Jouy-en-Josas, France [http://locus.jouy.inra.fr/cms/index.php?id=18 homepage]
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| − | [[Peter Graumann]], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]
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| − | =Your additional remarks=
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| − | =References=
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| − | <pubmed>18854156,,11222620,14651647,10844689 11222620 12060778 16461910 2987848 2168872 14651647, </pubmed>
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