CotX

• Description: spore coat protein (insoluble fraction)
  
  

Gene name	cotX
Synonyms
Essential	no
Product	spore coat protein (insoluble fraction)
Function	resistance of the spore
MW, pI	18 kDa, 4.228
Gene length, protein length	516 bp, 172 aa
Immediate neighbours	cotY, cotW
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU11760

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: spore wall (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: Q08313

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotV-cotW-cotX PubMed

cotX PubMed

• Sigma factor: before cotV: SigK PubMed

before cotX: SigK PubMed

• Regulation: repressed by casamino acids PubMed

• Regulatory mechanism: upstream of cotX: SpoIIID: repression PubMed, upstream of both promoters: GerE: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

H Ichikawa, L Kroos
Combined action of two transcription factors regulates genes encoding spore coat proteins of Bacillus subtilis.
J Biol Chem: 2000, 275(18);13849-55
[PubMed:10788508] [WorldCat.org] [DOI] (P p)

J Zhang, H Ichikawa, R Halberg, L Kroos, A I Aronson
Regulation of the transcription of a cluster of Bacillus subtilis spore coat genes.
J Mol Biol: 1994, 240(5);405-15
[PubMed:7519271] [WorldCat.org] [DOI] (P p)