yfjP

yfjP
168

similar to DNA-3-methyladenine glycosidase II

Locus
BSU_08010
Molecular weight
33.03 kDa
Isoelectric point
5.52
Protein length
287 aaSequenceBlast
Gene length
864 bpSequenceBlast
Function
unknown
Product
unknown
Essential
no
E.C.
3.2.2.21
Synonyms
yfjP
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0122 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
872,425  873,288
The protein
Catalyzed reaction/ biological activity
Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine (according to UniProt)
Protein family
alkylbase DNA glycosidase AlkA family (with AlkA, according to UniProt)
Structure
3OH6 (PDB) (from E. coli, corresponds to aa 68 ... 283, 27.4% identity) PubMed
O31544 (AlphaFold)
Expression and Regulation
Operons
Genes
yfjP-yfjO
Description
PubMed
Open in new tab

yfjP→yfjO

2025-03-17 00:37:38

Jstuelk

76

f0780656463c19550802ca69fcbe4c22787f1b22

5E8CA0DFBDB527F0A8E65EB56DDFD85DEB8250F8

Biological materials
Mutant
MGNA-C275 (yfjP::erm), available at the NBRP B. subtilis, Japan
BKE08010 (yfjP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAGATCCACCCTTTTTT,  downstream forward: _UP4_TAATTCGTCTGACAATGAAG
BKK08010 (yfjP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAGATCCACCCTTTTTT,  downstream forward: _UP4_TAATTCGTCTGACAATGAAG
References
Reviews
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Original Publications
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82CDC97D16426016CA7E75240C1A6D0D600F4375

Page visits: 1686

Time of last update: 2025-04-06 16:50:26

Author of last update: Jstuelk