rnjB
168
RNase J2
Locus
BSU_16780
Molecular weight
56.67 kDa
Isoelectric point
9.18
Function
RNA processing and degradation
Product
RNase J2
Essential
no
Synonyms
rnjB, ymfA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0595 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,749,418 → 1,751,085
Phenotypes of a mutant
growth advantage at low pressure and at 27°C at normal pressure (has been selected at low pressure) PubMed
The protein
Catalyzed reaction/ biological activity
endoribonuclease, involved in processing of ''thrS'' mRNA
Protein family
metallo-beta-lactamase superfamily (according to UniProt)
RNase J subfamily (according to UniProt)
Structure
3ZQ4 (PDB), RNase J1, 49% identity, 81% similarity PubMed
Paralogous protein(s)
cytoplasm, colocalizes with the ribosomes PubMed
Expression and Regulation
Operons
Biological materials
Mutant
MGNA-B114 (ymfA::erm), available at the NBRP B. subtilis, Japan
GP1113 (rnjB::miniTn10 spc), available in Jörg Stülke's lab
GP1291 (ΔrnjB::cat), available in Jörg Stülke's lab
Expression vectors
GP1687, chromosomal expression of rnjB-Strep::kan in B. subtilis, available in Jörg Stülke's lab
GP1724, chromosomal expression of rnjB-Strep::spc in B. subtilis, based on pGP1389, available in Jörg Stülke's lab
pGP1438: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab
pGP1440: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab, PubMed
FLAG-tag construct
GP1001 (spc, based on pGP1331), available in Jörg Stülke's lab
LacZ fusion
pGP419 (in pAC7), available in Jörg Stülke's lab
GFP fusion
GP1695 (in pHJS-105 PubMed), expression of rnjB-sfGFP::spc under a xylose-inducible promoter in B. subtilis, available in Jörg Stülke's lab PubMed
GP1699 (in pBP43), expression of rnjB-GFP::spc under the native promoter, available in Jörg Stülke's lab PubMed
Labs working on this gene/protein
Harald Putzer, IBPC Paris, France Homepage
References
Reviews
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Original Publications
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Time of last update: 2024-12-19 07:14:11
Author of last update: Jstuelk