rnjB

rnjB
168

RNase J2

Locus
BSU_16780
Molecular weight
56.67 kDa
Isoelectric point
9.18
Protein length
Gene length
Function
RNA processing and degradation
Product
RNase J2
Essential
no
Synonyms
rnjB, ymfA

Genomic Context

List of homologs in different organisms, belongs to COG0595 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon

Gene
Coordinates
1,749,418 → 1,751,085
Phenotypes of a mutant
growth advantage at low pressure and at 27°C at normal pressure (has been selected at low pressure) PubMed
The protein
Catalyzed reaction/ biological activity
endoribonuclease, involved in processing of ''thrS'' mRNA
Protein family
metallo-beta-lactamase superfamily (according to UniProt)
RNase J subfamily (according to UniProt)
Structure
3ZQ4 (PDB), RNase J1, 49% identity, 81% similarity PubMed
Paralogous protein(s)
cytoplasm, colocalizes with the ribosomes PubMed
Expression and Regulation
Operons
Genes
Description
Regulation
constitutive expression PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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rnjB

2024-12-08 06:18:11

ghost

67

66c326bcc376993734796ad2ba0768266f905477

09B108FF8F2780B6C9057C169FC47AF565F4E5BE

Biological materials
Mutant
MGNA-B114 (ymfA::erm), available at the NBRP B. subtilis, Japan
GP1113 (rnjB::miniTn10 spc), available in Jörg Stülke's lab
GP1291 (ΔrnjB::cat), available in Jörg Stülke's lab
BKE16780 (ΔrnjB::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CAAAATCTATATCCTCCTAG,  downstream forward: _UP4_TAATGACTGACTAAAGACCG
BKK16780 (ΔrnjB::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CAAAATCTATATCCTCCTAG,  downstream forward: _UP4_TAATGACTGACTAAAGACCG
Expression vectors
GP1687, chromosomal expression of rnjB-Strep::kan in B. subtilis, available in Jörg Stülke's lab
GP1724, chromosomal expression of rnjB-Strep::spc in B. subtilis, based on pGP1389, available in Jörg Stülke's lab
pGP1438: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab
pGP1440: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab, PubMed
FLAG-tag construct
GP1001 (spc, based on pGP1331), available in Jörg Stülke's lab
LacZ fusion
pGP419 (in pAC7), available in Jörg Stülke's lab
GFP fusion
GP1695 (in pHJS-105 PubMed), expression of  rnjB-sfGFP::spc under a xylose-inducible promoter in B. subtilis, available in Jörg Stülke's lab PubMed
GP1699 (in pBP43), expression of  rnjB-GFP::spc under the native promoter, available in Jörg Stülke's lab PubMed
Labs working on this gene/protein
Harald Putzer, IBPC Paris, France Homepage
References
Reviews
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Original Publications
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Time of last update: 2024-12-19 07:14:11

Author of last update: Jstuelk