yvdF

yvdF
168

glucan 1,4-alpha-maltohydrolase, neopullulanase, maltogenic amylase

Locus
BSU_34620
Molecular weight
68.68 kDa
Isoelectric point
5.18
Protein length
589 aaSequenceBlast
Gene length
1770 bpSequenceBlast
Function
starch and maltodextrin utilization
Product
glucan 1,4-alpha-maltohydrolase, neopullulanase, maltogenic amylase
Essential
no
Synonyms
yvdF, bbmA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0366 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,555,902  3,557,671
The protein
Protein family
glycosyl hydrolase 13 family (according to UniProt)
Structure
1J0J (PDB) (from G. stearothermophilus, 56% identity, complex with maltotetraose),  1J0H (PDB) (from G. stearothermophilus, 56% identity)
O06988 (AlphaFold)
Localization
on both sides of the cytoplasmic membrane and in the periplasm during vegetative growth but in the cytoplasm of prespores PubMed
Expression and Regulation
Operons
Genes
mdxR-yvdF-mdxE-mdxF-mdxG-yvdJ-yvdK-malL-pgcM
Description
PubMed
Regulation
induced in the presence of starch, β-cyclomaltodextrin, or maltose PubMed
Regulatory mechanism
MdxR: activation, PubMed, in mdxR regulon
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mdxR→pgcM

2025-04-04 20:01:18

Jstuelk

129

875DD090FAB06D126BF7206081D457B6A5E12492

6EDA4FDB63800BE601694138B9C35457B6878679

Biological materials
Mutant
MGNA-B628 (yvdF::erm), available at the NBRP B. subtilis, Japan
BKE34620 (yvdF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTCCCCCTTTGATTT,  downstream forward: _UP4_TAACATTCTGTTATCGGTAA
BKK34620 (yvdF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTCCCCCTTTGATTT,  downstream forward: _UP4_TAACATTCTGTTATCGGTAA
References
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A577441DE4ABAF624D507DB80FBED2A652BCF385

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Time of last update: 2025-04-01 03:59:00

Author of last update: Robert.warneke