rocA

rocA
168

3-hydroxy-1-pyrroline-5-carboxylate dehydrogenase

Locus
BSU_37780
Molecular weight
56.15 kDa
Isoelectric point
5.58
Protein length
515 aaSequenceBlast
Gene length
1548 bpSequenceBlast
Function
arginine, ornithine and citrulline utilization
Product
3-hydroxy-1-pyrroline-5-carboxylate dehydrogenase
Essential
no
E.C.
1.5.1.12
Synonyms
rocA, ipa-76d
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1012 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,878,966  3,880,513
Phenotypes of a mutant
the putC rocA double mutant is unable to grow with proline, arginine, citrulline, or ornithine as the single source of nitrogen PubMed
The protein
Catalyzed reaction/ biological activity
H2O + L-glutamate 5-semialdehyde + NAD+ --> 2 H+ + L-glutamate + NADH (according to UniProt)
Protein family
aldehyde dehydrogenase family (according to UniProt)
Structure
3RJL (PDB) (1-pyrroline-5-carboxylate dehydrogenase from Bacillus licheniformis, 68% identity, 88% similarity)
P39634 (AlphaFold)
Modification
phosphorylation on (Thr-2 OR Thr-4 OR Tyr-5) PubMed
Paralogous protein(s)
IolA, YwdH, GabD, YfmT, AldX, AldY, GbsA, PutC, YcbD, DhaS
Expression and Regulation
Operons
Genes
rocA-rocB-rocC
Description
PubMed
Regulation
expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
induced in the presence of arginine (AhrC) PubMed
induced in the presence of arginine, citrulline or ornithine (with ornithine as the molecular inducer) (RocR) PubMed
Regulatory mechanism
CodY: repression, PubMed, in codY regulon
RocR: activation, PubMed, in rocR regulon
AhrC: activation, PubMed, in ahrC regulon
AbrB: repression, PubMed, in abrB regulon
Sigma factors
SigL: sigma factor, PubMed, in sigL regulon
Additional information
expression of the rocA-rocB-rocC operon is increased upon depletion of nusA (resulting from increased ahrC expression) Reference
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rocA→rocC

2025-03-18 21:34:11

Jstuelk

141

2DC790ED3D8C4B63BB982C1F1D977B49CCE4042B

BCA34B2E2917A99583D79922F2E2CBC45D25A78E

Biological materials
Mutant
BKE37780 (rocA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGTAGTCCCCCTCGTG,  downstream forward: _UP4_TGATTTGAGAGAAATAAAAT
BKK37780 (rocA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGTAGTCCCCCTCGTG,  downstream forward: _UP4_TGATTTGAGAGAAATAAAAT
GP3727 (rocA::tet trpC2) available in Jörg Stülke's lab PubMed
GP3747 (rocA::aphA3 trpC2) available in in Jörg Stülke's lab PubMed
GP3398 (rocA::tet putC::aphA3 trpC2) available in in Jörg Stülke's lab PubMed
Expression vectors
pGP3792: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
References
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69838717DC6BB27864D88C282BF5BC7CC558BFD7

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Time of last update: 2025-04-15 05:59:58

Author of last update: Jstuelk