murQ
168

N-acetyl-muramic acid-6P etherase (cleaves lactate from N-acetylmuramic acid)

Locus

BSU_01700

Molecular weight

32.55 kDa

Isoelectric point

5.4

Protein length

304 aaSequenceBlast

Gene length

915 bpSequenceBlast

Function

cell wall turnover

Product

N-acetyl-muramic acid-6P etherase

Essential

Synonyms

murQ, ybbI

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

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Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2103 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

192,051 192,965

Phenotypes of a mutant

strongly reduced viability during prolonged incubation in stationary phase PubMed

The protein

Catalyzed reaction/ biological activity

H₂O + N-acetyl-D-muramate 6-phosphate --> (R)-lactate + N-acetyl-D-glucosamine 6-phosphate (according to UniProt)

Protein family

GCKR-like family (single member, according to UniProt)

Domains

SIS domain (aa 59-222) (according to UniProt)

Structure

4S12 (PDB) (from Yersinia enterocolitica, 51% identity)

Q45582 (AlphaFold)

Modification

phosphorylation on Ser-2 PubMed

Expression and Regulation

Operons

Genes

murQ-murR-murP-amiE-nagZ-namZ

Description

PubMed

Regulation

expressed in late exponential and early stationary phase PubMed

Regulatory mechanism

MurR: repression, PubMed, in murR regulon

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Biological materials

Mutant

MGNA-B952 (ybbI::erm), available at the NBRP B. subtilis, Japan

BKE01700 (murQ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAGGATGCCCCCTGTTT, downstream forward: _UP4_CATCCATGATAAGGAGAGAA

BKK01700 (murQ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAGGATGCCCCCTGTTT, downstream forward: _UP4_CATCCATGATAAGGAGAGAA

References

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Time of last update: 2025-04-09 08:20:26

Author of last update: Melvin.boenninger

murQ 168

murQ
168