gmuC

gmuC
168

glucomannan-specific permease of the phosphotransferase system, EIIC of the PTS

Locus
BSU_05830
Molecular weight
48.27 kDa
Isoelectric point
9.8
Protein length
442 aaSequenceBlast
Gene length
1329 bpSequenceBlast
Function
glucomannan uptake and phosphorylation
Product
glucomannan-specific lichenan-specific PTS, EIIC component
Essential
no
Synonyms
gmuC, ydhO
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1455 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
627,284  628,612
The protein
Protein family
PTS permease, lactose family PubMed
Domains
PTS EIIC domain type-3 (aa 5-411) (according to UniProt)
Structure
3QNQ (PDB) (EIIC component of diacetylchitobiose specific PTS from Bacillus cereus; 41% identity, 80% similarity) PubMed
O05507 (AlphaFold)
Paralogous protein(s)
LicC, YwbA
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
gmuB-gmuA-gmuC-gmuD-gmuR-gmuE-gmuF-gmuG
Description
PubMed
Regulation
induced by cellobiose (GmuR) PubMed
Regulatory mechanism
GmuR: repression, PubMed, in gmuR regulon
CcpA: repression, PubMed, in ccpA regulon
AbrB: activation, PubMed, in abrB regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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gmuB→gmuG

2025-03-16 15:57:23

Jstuelk

109

1716709ba10a9af523abc0d89eb44ea784dd12d9

42FBC6CD7712CC913DB98C4B417CB4A13C8FAE20

Additional information
translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
Biological materials
Mutant
MGNA-C190 (ydhO::erm), available at the NBRP B. subtilis, Japan
BKE05830 (gmuC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GAACACCCTATCCACCCCGA,  downstream forward: _UP4_ATGTGACATAAGGGGAGAGA
BKK05830 (gmuC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GAACACCCTATCCACCCCGA,  downstream forward: _UP4_ATGTGACATAAGGGGAGAGA
References
Cao Y, Jin X, Levin EJ, Huang H, Zong Y, Quick M, Weng J, Pan Y, Love J, Punta M, Rost B, Hendrickson WA, Javitch JA, Rajashankar KR, Zhou M Crystal structure of a phosphorylation-coupled saccharide transporter. Nature. 2011 May 05; 473(7345):50-4. doi:10.1038/nature09939. PMID:21471968
Chumsakul O, Takahashi H, Oshima T, Hishimoto T, Kanaya S, Ogasawara N, Ishikawa S Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation. Nucleic acids research. 2011 Jan; 39(2):414-28. doi:10.1093/nar/gkq780. PMID:20817675
Sadaie Y, Nakadate H, Fukui R, Yee LM, Asai K Glucomannan utilization operon of Bacillus subtilis. FEMS microbiology letters. 2008 Feb; 279(1):103-9. doi:10.1111/j.1574-6968.2007.01018.x. PMID:18177310
Reizer J, Bachem S, Reizer A, Arnaud M, Saier MH, Stülke J Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis. Microbiology (Reading, England). 1999 Dec; 145 ( Pt 12):3419-29. . PMID:10627040

76324E0A6CAA8E1DD0B0C6FAE1CAB402231BF7CC

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Time of last update: 2025-04-09 05:52:26

Author of last update: Melvin.boenninger